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相关概念视频

Ribosome Profiling02:24

Ribosome Profiling

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Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
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RACE - Rapid Amplification of cDNA Ends02:35

RACE - Rapid Amplification of cDNA Ends

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Rapid Amplification of cDNA Ends, or RACE, is one of the most effective methods to obtain a full-length cDNA from an mRNA sequence between a known internal region to the unknown sequence at the 5’ or 3’ end. The unknown region is cloned in the cDNA by a gene-specific primer that binds the known end, and a hybrid primer that attaches a predefined anchor sequence to the unknown end of the cDNA. The sequence in between is amplified by PCR with an anchor primer and a gene-specific...
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相关实验视频

Updated: May 30, 2025

Transcriptome Profiling of In-Vivo Produced Bovine Pre-implantation Embryos Using Two-color Microarray Platform
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一个全面的等位基因特异表达资源,用于马类转录组.

Harrison D Heath1, Sichong Peng1,2, Tomasz Szmatola1,3

  • 1Department of Population Health and Reproduction, Davis School of Veterinary Medicine, University of California, Room 4206 Vet Med3A One Shields Ave, Davis, CA, 95616, USA.

BMC genomics
|January 30, 2025
PubMed
概括
此摘要是机器生成的。

基因特异性表达 (ASE) 分析揭示了马基因组中的调控变异. 这项研究确定了显著的ASE事件,特别是在肝脏组织中,为马类基因调节提供了洞察力.

关键词:
表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.范说,这是一个很好的选择.哈普洛型是指一种类型.马马的马,就是马马.通过RNA测序进行RNA测序.

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Analysis of Chromosome Segregation, Histone Acetylation, and Spindle Morphology in Horse Oocytes
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Identification of Key Factors Regulating Self-renewal and Differentiation in EML Hematopoietic Precursor Cells by RNA-sequencing Analysis
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Identification of Key Factors Regulating Self-renewal and Differentiation in EML Hematopoietic Precursor Cells by RNA-sequencing Analysis

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相关实验视频

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Analysis of Chromosome Segregation, Histone Acetylation, and Spindle Morphology in Horse Oocytes
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Identification of Key Factors Regulating Self-renewal and Differentiation in EML Hematopoietic Precursor Cells by RNA-sequencing Analysis
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科学领域:

  • 基因组学就是基因组学.
  • 分子生物学分子生物学
  • 动物科学动物科学

背景情况:

  • 基因特异性表达 (ASE) 分析为控制基因表达的 cis 调节机制提供了详细的见解.
  • 了解ASE对于破译基因调节复杂性至关重要.

研究的目的:

  • 在马体组织中进行全面的等位基特异性表达分析.
  • 为了识别和描述马匹的等位基因表达事件.
  • 为了研究基因失衡的组织特异性模式.

主要方法:

  • 综合分析Iso-seq和短读RNA测序数据从四个纯血统马跨越九个组织.
  • 使用从长期读取的测序数据中得出的单元类型来量化等位基表达.
  • 在较大的马肝样本队列中验证已识别的等位基因特异性变异.

主要成果:

  • 对42,900个等位基因表达事件的比较揭示了635个 (1.48%) 的ASE病例.
  • 肝脏组织表现出最高比例的ASE事件.
  • 与ASE相关的遗传变异经常位于基因组修饰区域内 (64.2%).
  • 验证证实了在97%的测试过的等位基因特定变异中出现了ASE.

结论:

  • 这项研究为马类基因调节研究产生了有价值的,公开可访问的资源.
  • 结果强调了马体基因组中标基因失衡的显著组织特异性.
  • 这项工作有助于未来对马体组织内的调控变异进行调查.