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通过蛋白质组学和代谢组学探索的新型预测性生物标志物用于心脏性产后出血

Jiangxue Qu^1,2,3, Hai Jiang^1,2,3, Huifeng Shi^1,2,3

¹Department of Obstetrics and Gynecology, National Clinical Research Center for Obstetrics and Gynecology, Peking University Third Hospital, Peking University Third Hospital), National Center for Healthcare Quality Management in Obstetrics, Beijing, 100191, China.

BMC pregnancy and childbirth

|January 30, 2025

概括

这项研究确定了宫性阴道液 (CVF) 中的关键生物标志物,用于预测由子宫动引起的产后出血 (PPH). 希斯蒂丁,DPYD,CD163和FGL2在早期检测和管理PPH方面表现有前途.

科学领域:

发现生物标志物的发现.
生殖医学是一种生殖医学.
奥米克斯技术的技术.

背景情况:

产后出血 (PPH) 是全球主要的孕产妇死亡原因,子宫缩约占70%的病例.
目前对无动性PPH缺乏预测方法,这阻碍了及时的临床干预.
这项研究的目的是利用综合的奥米克方法来确定性PPH的预测生物标志物.

研究的目的:

使用宫性阴道液 (CVF) 的代谢和蛋白质组分析,选潜在的可预测性生物标志物.
验证已识别的代谢物和蛋白质,并评估它们对阳性PPH的预测能力.

主要方法:

通过LC-MS/MS收集了来自女性的CVF样本,用于通过LC-MS/MS进行代谢和蛋白质分析.
使用质谱和ELISA验证了显著不同的代谢物和蛋白质.
采用多变量逻辑回归并计算曲线下的面积 (AUC) 来评估生物标志物的预测性能.

主要成果:

在PPH和对照组之间,在β-alanine代谢途径中的代谢物和蛋白质中发现了显著的差异.
在PPH组中观察到静丁和二皮里米丁脱酶 (DPYD) 的下调.
在患有PPH的女性中发现了低度的histidine,DPYD和与免疫相关的蛋白质 (CD163,FGL2). 组合生物标志物在PPH预测中达到0.964的AUC.

结论:

免疫炎症似乎在PPH的发展中起作用.
在CVF中,histidine,DPYD,CD163和FGL2与子宫有关.
这些已识别的生物标志物显示出预测无氧PPH的潜力,从而使早期的管理策略成为可能.

关键词:

产后血栓性出血产后血栓性出血产妇死亡率产妇死亡率这是一个代谢学 (metabonomic) 体系.预测预测预测蛋白质基的蛋白质基体是什么

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