老化后 ортодонтических带的细胞毒性和基因毒性:一个体外研究研究
Maryam Karandish1, Bahar Khademi1, Golnaz Ziaei1
1Orthodontic Research Center, School of Dentistry, Shiraz University of Medical Sciences, Shiraz, Iran.
BMC oral health
|January 30, 2025
概括
美国正统牙科 (AO) 带在酸性条件下表现出细胞毒性和基因毒性,与3S牙科带不同. 在酸性环境中,AO带对牙细胞有毒,而在中性条件下,这两个带都具有生物相容性.
科学领域:
- 生物材料科学 生物材料科学
- 牙科材料 牙科材料
- 细胞生物学 细胞生物学
背景情况:
- ортодонтические带通常用于牙科治疗.
- ортодонтических带的生物相容性对于患者安全至关重要.
- 老化和pH等环境因素会影响牙科材料的性能.
研究的目的:
- 评估两个正统牙大带的细胞毒性和基因毒性作用.
- 评估老化在酸性和中性人工唾液对这些频段的影响.
- 为了比较美国正统牙科 (AO) 和3S牙科带的生物相容性.
主要方法:
- 使用热循环和暴露在pH4.5和pH7.4.4的人工唾液下,两种商业正义牙牙带 (AO和3S-dental) 经过了老化.
- 用MTT测定对人类牙纤维细胞样 (HGF1-PI 1) 细胞系进行了细胞毒性评估.
- 用彗星测定来检测DNA损伤来评估基因毒性.
主要成果:
- 在酸性条件下 (pH4.5),但在中性条件下 (pH7.4) 没有显著的细胞毒性.
- 在酸性或中性pH下,3S牙牙带没有显著的细胞毒性作用.
- 在pH值为4.5的两种品牌中都观察到遗传毒性,AO带显示的DNA损伤 (彗星尾巴较长) 比3S牙带更大. AO带在中性pH下也显示了一些DNA破坏,而3S牙带没有.
结论:
- AO 带显示出细胞毒性和基因毒性,特别是在酸性环境中.
- 3S牙牙带表现出更好的生物相容性,没有细胞毒性作用,基因毒性较低.
- 这两种波段在中性条件下都是生物相容的,但AO波段在酸性口腔环境中可能对牙细胞构成风险.
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