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相关概念视频

RNA Splicing01:32

RNA Splicing

55.9K
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
55.9K
Alternative RNA Splicing02:18

Alternative RNA Splicing

20.9K
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
20.9K
Experimental RNAi02:15

Experimental RNAi

6.0K
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
6.0K
Chromatin Structure Regulates pre-mRNA Processing02:41

Chromatin Structure Regulates pre-mRNA Processing

6.9K
In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
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Pre-mRNA Processing: RNA Splicing01:36

Pre-mRNA Processing: RNA Splicing

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RNA Interference01:23

RNA Interference

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RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
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Using the E1A Minigene Tool to Study mRNA Splicing Changes
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向RNA剪接调制:抗癌策略的新视角?

Xuemei Lv1,2, Xiaoyu Sun1, Yang Gao1

  • 1Department of Pharmacology, School of Pharmacy, China Medical University, No.77 Puhe Road, Shenyang North New Area, Shenyang, Liaoning Province, 110122, P. R. China.

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概括

异常的RNA拼接驱动癌症的发展. 本综述探讨了癌症特异性拼接特征和RNA拼接向治疗方法,包括拼接切换抗意义寡核酸 (ASO),以改善癌症治疗.

关键词:
另一个替代拼接.癌症 癌症 癌症 癌症拼接切换的ASO是一种ASO.拼接调制器 拼接调制器有针对性的治疗.

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科学领域:

  • 分子生物学分子生物学
  • 遗传学 是一个遗传学.
  • 在瘤学瘤学.

背景情况:

  • 替代RNA拼接可以从单个基因中产生多种不同的蛋白质.
  • 异常RNA拼接是大多数人类癌症的标志.
  • 向拼接为癌症治疗提供了新的治疗策略.

研究的目的:

  • 为了审查癌症转录组中的拼接改变的签名.
  • 讨论剪接异常在瘤发生和进展中的作用.
  • 突出RNA拼接向癌症疗法及其临床转化.

主要方法:

  • 关于RNA拼接和癌症的科学文献的综述.
  • 对癌细胞转录组数据进行分析,以检测剪接变化.
  • 关于药理学药剂和拼接切换抗意义寡核酸 (ASO) 的讨论.

主要成果:

  • 在各种癌症类型中识别出明显的拼接改变的签名.
  • 详细介绍了拼接异常对癌症发病和转移的贡献.
  • 总结了拼接向治疗的机制和临床进展.

结论:

  • 异常RNA拼接是癌症的一个关键驱动因素.
  • 包括ASOs在内的RNA拼接向策略,在癌症治疗方面表现有前途.
  • 需要进一步的研究来克服临床翻译方面的挑战.