叶子提取物从Trichilia emetica的抗菌和抗真菌活动
Lydia Chenesai Mugayi1, Stanley Mukanganyama2
1Department of Biotechnology and Biochemistry, University of Zimbabwe, Mount Pleasant, Harare, Zimbabwe.
Scientifica
|January 31, 2025
概括
来自Trichilia emetica的叶子提取物显示出有前途的抗菌和抗真菌特性,可以对抗疹和菌根菌. 这些天然化合物可能为传染病提供替代治疗方法,可能通过破坏微生物细胞膜.
科学领域:
- 自然产品化学 自然产品化学
- 微生物学 微生物学
- 药理学 药理学是指药理学的学科.
背景情况:
- 由疹菌和菌根菌等微生物引起的传染病给全球健康带来了重大挑战.
- 抗微生物药物耐药性正在升级,需要寻找新的治疗剂.
- 植物衍生化合物是对抗病原微生物的替代治疗方法的一个有希望的来源.
研究的目的:
- 评估Trichilia emetica各种叶子提取物的抗菌和抗真菌活性.
- 为了确定针对Mycobacterium smegmatis,Mycobacterium aurum,Candida tropicalis和Candida albicans的最小抑制度 (MIC),我们需要测量这些度.
- 研究活性提取物对微生物生长和细胞完整性的作用方式.
主要方法:
- 使用溶剂制备Trichilia emetica的叶片提取物,包括,乙烯酸乙烯,乙,DCM,甲醇,乙醇,水和70%的乙醇水溶液.
- 使用微乳汁稀释试验来确定针对目标生物的MIC值.
- 细胞壁完整性通过sorbitol测定进行评估,通过测量核酸和蛋白质泄漏来评估膜完整性.
主要成果:
- 乙烯酸乙烯和70%的乙醇水性提取物表现出最强烈的活性,MICs在125~1000μg/mL之间.
- 这些强效的提取物在位醇的存在下并没有抑制Candida tropicalis的生长,这表明细胞壁完整性不是主要的目标.
- 提取物诱导了Candida tropicalis和Mycobacterium smegmatis中的核酸和蛋白质的泄漏,但不是Mycobacterium aurum,这表明膜破坏是可能的机制.
结论:
- 特里奇利亚喷植物的叶子提取物具有显著的抗菌菌和抗真菌活性.
- 观察到的抗微生物效应很可能是由于微生物细胞膜的破坏,导致重要的细胞成分的泄漏.
- 三菌 (Trichilia emetica) 代表了开发新抗菌剂的潜在来源,以对抗耐药性病原体.
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