相关实验视频

Updated: May 30, 2025

Agrobacterium-Mediated Genetic Transformation, Transgenic Production, and Its Application for the Study of Male Reproductive Development in Rice

Agrobacterium-Mediated Genetic Transformation, Transgenic Production, and Its Application for the Study of Male Reproductive Development in Rice

Published on: October 6, 2020

在CTF1中的自然变异,在水的开花阶段赋予寒冷耐受性

Jingfang Dong¹, Shaohong Zhang¹, Haifei Hu¹

¹Rice Research Institute, Key Laboratory of Genetics and Breeding of High Quality Rice in Southern China (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Guangdong Key Laboratory of New Technology in Rice Breeding, Guangdong Rice Engineering Laboratory, Guangdong Academy of Agricultural Sciences, Guangzhou, China.

Plant biotechnology journal

|January 31, 2025

概括

研究人员发现了一种新的基因,CTF1,对于改善花期 (CTF) 米耐寒性至关重要. 这一发现为分子育种提供了一种新型基因,以提高作物弹性并最大限度地减少产量损失.

科学领域:

植物遗传学植物遗传学
农作物科学农作物科学
分子育种是一种分子育种.

背景情况:

提高水在开花阶段 (CTF) 的耐寒性,对于防止产量损失至关重要.
由于特征的复杂性和长时间的研究时间表,研究CTF的遗传基础具有挑战性.
之前没有对CTF进行过精细映射或克隆的特定QTL.

研究的目的:

识别和克隆负责米在开花阶段耐寒的基因.
开发分子育种策略,以提高大米品种的CTF.
研究CTF相关基因的遗传变异和进化史.

主要方法:

使用特定大米品种的单段替代线路 (SSSL) 的建设.
基于地图的克隆,以识别潜在的显著QTL的因果基因.
对遗传变异的分析,哈普洛型的表征,以及对大米加入的选择分析.

主要成果:

在大米中发现了两种适应寒冷的QTL,qCTF3和qCTF6.
导致基因CTF1,QCTF6的基因,已成功克隆.
编码和促进区域的CTF1变异影响CTF;在日本大米中正在选择一个特定的哈普洛型.

结论:

CTF1是一种新型基因,有利于改善耐寒性大米的分子育种.
在CTF1中的遗传变异,包括新生成的突变,有助于耐寒性.
CTF1对推进旨在增强作物弹性米育种计划具有重大潜力.

关键词:

在 QTL 时段,你会得到 QTL.寒冷耐受性耐寒性耐寒性功能网站的功能网站.单种类型分析分析.米米饭米饭米饭米饭.一个单段替代线路的替代线路.

更多相关视频

Transverse Sectioning of Mature Rice Oryza sativa L. Kernels for Scanning Electron Microscopy Imaging Using Pipette Tips as Immobilization Support

Transverse Sectioning of Mature Rice Oryza sativa L. Kernels for Scanning Electron Microscopy Imaging Using Pipette Tips as Immobilization Support

Published on: January 25, 2022

Identifying Mutations by High Resolution Melting in a TILLING Population of Rice

Identifying Mutations by High Resolution Melting in a TILLING Population of Rice

Published on: September 2, 2019

相关概念视频

Responses to Heat and Cold Stress

Responses to Heat and Cold Stress

Every organism has an optimum temperature range within which healthy growth and physiological functioning can occur. At the ends of this range, there will be a minimum and maximum temperature that interrupt biological processes.

Trihybrid Crosses

Trihybrid Crosses

Trihybrid Crosses
Some of Mendel’s crosses examined three pairs of contrasting characteristics. Such a cross is called a trihybrid cross. A trihybrid cross is a combination of three individual monohybrid crosses. For example, plant height (tall vs. short), seed shape (round vs. wrinkled), and seed color (yellow vs. green).
The F1 generation plants of a trihybrid cross are heterozygous for all three traits and produce eight gametes. Upon self-fertilization, these gametes have an equal...

Monohybrid Crosses

Monohybrid Crosses

Plant Breeding and Biotechnology

Plant Breeding and Biotechnology

Crop cultivation has a long history in human civilization, with records showing the cultivation of cereal plants beginning at around 8000 BC. This early plant breeding was developed primarily to provide a steady supply of food.

Dihybrid Crosses

Dihybrid Crosses

Chi-square Analysis

Chi-square Analysis

The chi-square test is a statistical hypothesis test. It is used to check whether there is a significant difference between an expected value and an observed value. In the context of genetics, it enables us to either accept or reject a hypothesis, based on how much the observed values deviate from the expected values.
The chi-square test was developed by Pearson in 1990.
The first step of performing a Chi-square analysis is to establish a null hypothesis, which assumes that there is no real...