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在Arabidopsis的干旱压力期间,HOS15会影响DIL9蛋白质的稳定性

Shah Zareen^1,2, Akhtar Ali^1,2,3, Junghoon Park^1,2

¹Department of Biomedical Science & Engineering, Konkuk University, Seoul, 05029, South Korea.

The New phytologist

|January 31, 2025

概括

高表达的透反应基因15 (HOS15) 降解干旱诱导的Like 19 (DIL9) 蛋白质,影响干旱耐受性. 压力条件稳定了HOS15-DIL9的相互作用,促进了DIL9的降解,增强了植物的生存.

科学领域:

植物生物学植物生物学
分子生物学分子生物学
遗传学是一个遗传学.

背景情况:

高表达的透反应基因15 (HOS15) 负面调节干旱耐受性.
控制HOS15关键转录调节者的精确机制尚未完全理解.

研究的目的:

研究HOS15在干旱反应因子调节蛋白质稳定性的作用.
在正常和应激条件下阐明HOS15和干旱诱导的Like 19 (DIL9) 之间的相互作用.

主要方法:

同免疫沉测试以确认蛋白质与蛋白质相互作用.
亚酸 (ABA) 处理和蛋白质体降解试验.
对hos15-2和dil9/hos15双突变的遗传分析.
染色体免疫沉 (ChIP) 测试用于评估促进体结合.

主要成果:

HOS15在物理上与DIL9相互作用,并针对DIL9进行蛋白质体降解.
酸 (ABA) 处理增强了HOS15-DIL9的相互作用,促进了DIL9的降解.
DIL9 作用于 HOS15 的下游; DIL9 的丧失会损害 hos15-2 的干旱耐受性.
DIL9与ABF转录因子促进体结合,增强其表达和ABA依赖的干旱耐受性.

结论:

在正常情况下,HOS15会降解DIL9.
干旱或ABA压力稳定了HOS15-DIL9相互作用,导致DIL9降解.
压力诱导的DIL9活动促进了ABF表达,增强了植物的干旱耐受性.

关键词:

亚巴和干旱压力ABA和干旱压力在ABFs TFs中使用.阿拉比多普西斯 (Arabidopsis) 是一种植物.在 DIL9 中,我们可以使用 DIL9 .这就是HOS1515的原因.蛋白质群体的降解

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