相关实验视频
Updated: May 30, 2025

Techniques to Induce and Quantify Cellular Senescence
Published on: May 1, 2017
炭基终端调节蛋白诱导细胞衰老,并通过Zic2在老鼠中随着衰老而升级
Weiran Shan1, Jun Li1, Zachary Philpot1,2
1Department of Anesthesiology, University of Virginia, Charlottesville, Virginia, USA.
碳素终端调节蛋白 (CTMP) 随着年龄的增长而增加,并诱导细胞衰老. 指蛋白Zic2抑制CTMP,其与年龄相关的下降有助于大脑中CTMP水平升高.
科学领域:
- 神经科学是一个神经科学.
- 细胞生物学 细胞生物学
- 衰老研究研究 衰老研究
背景情况:
- 碳基终端调节蛋白 (CTMP) 涉及到各种生理和病理过程.
- 以前的研究表明,CTMP随着年龄的增长而增加,并影响大脑缺血性耐受性.
- 衰老期间CTMP表达的调节及其对细胞衰老的影响仍然不清楚.
研究的目的:
- 为了研究CTMP表达与衰老的调节机制.
- 为了确定CTMP水平改变对细胞衰老的影响.
- 阐明指蛋白Zic2 (Zic2) 在CTMP调节中的作用.
主要方法:
- 细胞中稳定的CTMP过度表达以评估衰老生物标志物.
- 在Fischer 344雄性老鼠大脑中进行西斑和免疫染色检测,以检测CTMP和Zic2.
- 促进者活动测试检查Zic2对CTMP表达的调节.
主要成果:
- 过度表达CTMP增强了衰老标志物 (例如,β-galactosidase染色) 和减少了细胞增殖.
- 在老鼠大脑中,Zic2表达随着年龄的增长而下降,其中Zic2和CTMP都在神经元中表达.
- 发现Zic2可以结合CTMP基因促进体片段并抑制其活性,在过度表达时降低CTMP水平.
结论:
- CTMP诱导了细胞衰老.
- Zic2作为CTMP表达的抑制剂.
- 与年龄相关的Zic2下降有助于在衰老过程中增加CTMP水平.
更多相关视频
13:59A Quantitative Measurement of Reactive Oxygen Species and Senescence-associated Secretory Phenotype in Normal Human Fibroblasts During Oncogene-induced Senescence
Published on: August 12, 2018
08:52Measurement of Protein Turnover Rates in Senescent and Non-Dividing Cultured Cells with Metabolic Labeling and Mass Spectrometry
Published on: April 6, 2022
相关概念视频
PI3K/mTOR/AKT Signaling Pathway
Abnormal Proliferation
Negative Regulator Molecules
mTOR Signaling and Cancer Progression
The mTOR pathway or the...
Calmodulin-dependent Signaling
The Ca2+-CaM complex does not have enzymatic activity by itself. Instead, the complex binds downstream target proteins, including membrane proteins or enzymes,...
Molecular Factors Affecting Cell Division
Several proteins function as internal regulators to ensure each cell cycle stage is completed faithfully before proceeding to the next. Regulator molecules may act directly or influence the activity or production of other...