候选基因SibHLHA调节香素驱动的紫色色素在芝麻花中的紫色
Fengli Zhao1,2, Chengqi Cui1,2, Wenxing Wei1
1Henan Sesame Research Center, Henan Academy of Agricultural Sciences, Zhengzhou, China.
概括
研究人员确定SibHLHA基因对芝麻的紫色花色至关重要. 在Sibhlha的一种特定突变减少了安东,影响了花的色素,并为开发新的芝麻品种提供了洞察力.
科学领域:
- 植物遗传学 植物遗传学
- 分子生物学分子生物学
- 农业科学 农业科学
背景情况:
- 安西是花朵中重要的颜料,影响着授粉和耐压力.
- 芝麻 (Sesamum indicum) 的花色调节的遗传基础在很大程度上仍然没有被描述.
研究的目的:
- 为了阐明控制芝麻花颜色的分子机制.
- 在芝麻中识别负责紫色花色素化的基因.
主要方法:
- 从紫花和白花的芝麻线交叉构建一个F2种群.
- 大量分离分析测序 (BSA-seq) 和竞争性等位基因特定PCR (KASP) 标记分析用于基因映射.
- 基因表达概况,功能注释和序列分析以确定候选基因.
主要成果:
- 芝麻中的紫色花色是由位于6号染色体上的单个位点SiFC控制的.
- SibHLHA (芝麻10992) 被确定为SiFC的最可能的候选基因.
- 白花变体Sibhlha的第二个外体中的单核酸多态 (SNP) 通过影响蛋白质相互作用来破坏安索亚宁积累.
结论:
- SibHLHA在调节氨酸合成,从而调节芝麻冠状颜色方面发挥着关键作用.
- 了解SibHLHA的功能为为装饰和消费用途培育具有所需花色的芝麻品种提供了基础.
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