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通过Lacticaseibacillus rhamnosus LRH30进行生物质和外聚糖类生产的统计优化和分析
Helena Mylise Copeland1,2,3, Susan Maye4, George MacLeod5,6,4
1School of Biotechnology, Dublin City University, Dublin, D9, Ireland. helena.copeland@dcu.ie.
World journal of microbiology & biotechnology
|January 31, 2025
概括
乳酸糖菌 (Lacticaseibacillus rhamnosus LRH30) 产生具有抗炎性质的外聚糖 (EPS). 优化生物反应器中的温度和空气流量可以提高生物质和EPS产量,从而作为天然食品添加剂的潜在用途.
科学领域:
- 生物技术和食品科学 生物技术和食品科学
- 微生物学 微生物学
- 免疫学 免疫学 免疫学
背景情况:
- 乳酸细菌外聚糖 (EPS) 由于其免疫调节潜力,显示出其作为天然食品添加剂的前景.
- 乳酸菌cillus rhamnosus LRH30是一种有兴趣的菌株,用于生产生物活性EPS.
- 优化生物过程参数对于最大限度地提高生物质和EPS的产量至关重要.
研究的目的:
- 为了优化温度和空气流量,从Lacticaseibacillus rhamnosus LRH30中获得高产量的生物质和外聚糖 (EPS).
- 评估L. rhamnosus LRH30及其产生的EPS的免疫调节潜力.
- 评估优化生产条件的可扩展性.
主要方法:
- 中央复合材料设计用于优化250毫升生物反应器中的温度和空气流量.
- 在优化条件下测量了生物质和EPS产量.
- 扩大规模的实验是在2L生物反应器中进行的.
- 福里埃变换红外光谱法 (FTIR) 用于EPS的表征.
- 使用J774A.1小鼠巨细胞和细胞因子/化学因子分泌分析来评估免疫调节作用.
主要成果:
- 一个二次模型有效地描述了生物质和EPS生产.
- 生物质的最佳条件是37.01°C和0.12vm的空气流;对于EPS,20.1°C和0.18vm的空气流.
- 小规模优化生产的产量为10.1 g/L生物质和520.2 mg/LEPS.
- 在2L反应堆中的扩展导致生物质8.54g/L和EPS654.6mg/L,显示出EPS产量增加.
- 鉴定证实了EPS的多糖性质.
- L. rhamnosus LRH30及其EPS显示主要具有抗炎作用.
结论:
- 生物处理优化对L. rhamnosus LRH30中的生物质和EPS生产产生了重大影响.
- 扩大规模的研究表明,以更大的数量提高EPS收益率.
- 由L. rhamnosus LRH30产生的EPS表现出有希望的抗炎性质.
- 这些发现支持L. rhamnosus LRH30 EPS作为功能性食品成分的潜力.
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