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相关实验视频

Updated: May 30, 2025

Author Spotlight: Elucidating the Dynamics of Mechano-Transduction and Nuclear Agitation in Mouse Oocytes
05:43

Author Spotlight: Elucidating the Dynamics of Mechano-Transduction and Nuclear Agitation in Mouse Oocytes

Published on: January 12, 2024

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在成熟的小鼠卵细胞中图像,细分和量化皮质收缩的协议.

Gaëlle Letort1, Elvira Nikalayevich2

  • 1Department of Developmental and Stem Cell Biology, Institut Pasteur, CNRS UMR 3738, Université Paris Cité, 25 Rue du Dr. Roux, 75015 Paris, France.

STAR protocols
|January 31, 2025
PubMed
概括

缺少分支性动因的小鼠卵细胞在半球变化I过程中表现出延长的皮质收缩. 这项研究详细介绍了一种使用时差成像和ImageJ插件观察和分析这些收缩的协议.

关键词:
细胞培养培养的细胞培养.发育生物学是发展生物学.显微镜 显微镜是指使用显微镜.一个单细胞的单细胞.

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相关实验视频

Last Updated: May 30, 2025

Author Spotlight: Elucidating the Dynamics of Mechano-Transduction and Nuclear Agitation in Mouse Oocytes
05:43

Author Spotlight: Elucidating the Dynamics of Mechano-Transduction and Nuclear Agitation in Mouse Oocytes

Published on: January 12, 2024

677
Evaluation of the Spindle Assembly Checkpoint Integrity in Mouse Oocytes
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科学领域:

  • 细胞生物学 细胞生物学
  • 发展生物学 发展生物学
  • 生物物理学的生物物理.

背景情况:

  • 动氨酸的动态对于细胞分裂至关重要.
  • 卵细胞中的皮层收缩调节了介质进展.
  • 在卵细胞机制中分支性actin的作用尚未完全理解.

研究的目的:

  • 提出观察小鼠卵细胞皮质收缩的方案.
  • 分析actin结构对介质分裂的影响.
  • 提供一种量化皮层波动的方法.

主要方法:

  • 收集的prophase我逮捕了小鼠卵细胞.
  • 在转光照明中进行时间间隔成像,没有标记.
  • 使用ImageJ插件 (Oocytor和Radioak) 进行图像分析,用于细分和波动测量.

主要成果:

  • 缺少分支动蛋白的卵细胞表现出长时间的皮质收缩.
  • 该协议允许对环境事件进行无标签的观察.
  • 通过图像分析来实现皮层波动的量化.

结论:

  • 分支性活性蛋白对于调节小鼠卵细胞化I期间皮质收缩的持续时间至关重要.
  • 该协议有助于研究卵细胞机制和细胞分裂.
  • 这些发现有助于了解半体变异的生物物理调节.