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相关概念视频

Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

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Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
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Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

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Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
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相关实验视频

Updated: May 30, 2025

Fluorescence Lifetime Macro Imager for Biomedical Applications
06:01

Fluorescence Lifetime Macro Imager for Biomedical Applications

Published on: April 7, 2023

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多光谱激光扫描脉冲采样光寿命系统用于大规模组织成像.

Xuhui Liu, Julien Bec, Xiangnan Zhou

    Optics letters
    |January 31, 2025
    PubMed
    概括

    一个新的多光谱激光扫描脉冲采样光终身成像 (LSPS-FLIm) 系统能够快速,高分辨率的成像大组织样本. 这种先进的LSPS-FLIm技术为生物和临床应用提供了厘米尺度的视野.

    科学领域:

    • 生物医学光学 生物医学光学
    • 光成像技术 光成像技术
    • 显微镜的使用方法

    背景情况:

    • 光终身成像 (FLIm) 对于分析生物组织至关重要.
    • 现有的FLIM系统通常在视野,分辨率或速度方面面临限制.
    • 需要先进的成像系统,能够高细节地检查大型组织样本.

    研究的目的:

    • 开发和描述一种新的多光谱激光扫描脉冲采样光终身成像 (LSPS-FLIm) 系统.
    • 为了使大型组织样本能够快速,高分辨率的成像.
    • 在各种生物样本中评估系统的多功能性和性能.

    主要方法:

    • 实施多光谱激光扫描脉冲采样光终身成像系统.
    • 系统视野 (6 × 15 cm2) 和空间分辨率 (~17.5 μm) 的表征.
    • 用各种光染料,内源性组织光体和各种组织样本进行测试.

    主要成果:

    • LSPS-FLIm系统实现了高空间分辨率的厘米尺度成像.
    • 在解决各种组织类型的形态和分子特征方面表现出多功能性.
    • 在组织样本中成功区分了复杂的微观结构.

    更多相关视频

    Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells
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    Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells

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    Author Spotlight: Standardizing Spheroid Formation Methods for Metabolic and Oxygenation Analysis Using Fluorescence Lifetime Imaging Microscopy
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    Author Spotlight: Standardizing Spheroid Formation Methods for Metabolic and Oxygenation Analysis Using Fluorescence Lifetime Imaging Microscopy

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    808

    相关实验视频

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    Fluorescence Lifetime Macro Imager for Biomedical Applications

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    681
    Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells
    09:45

    Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells

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    Author Spotlight: Standardizing Spheroid Formation Methods for Metabolic and Oxygenation Analysis Using Fluorescence Lifetime Imaging Microscopy
    08:43

    Author Spotlight: Standardizing Spheroid Formation Methods for Metabolic and Oxygenation Analysis Using Fluorescence Lifetime Imaging Microscopy

    Published on: August 9, 2024

    808

    结论:

    • 开发的LSPS-FLIm系统为成像大型组织样本提供了重大进步.
    • 它提供高质量的成像,采集速度快,组织损伤最小.
    • 对于未来的生物和临床应用来说,LSPS-FLIm非常有前途.