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相关概念视频

Computed Tomography01:10

Computed Tomography

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Tomography refers to imaging by sections. Computed tomography (CT) is a non-invasive imaging technique that uses computers to analyze several cross-sectional X-rays to reveal minute details about structures in the body.
The technique was invented in the 1970s and is based on the principle that as X-rays pass through the body, they are absorbed or reflected at different levels. In the technique, a patient lies on a motorized platform while a computerized axial tomography (CAT) scanner rotates...
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Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

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Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
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Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

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Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
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Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

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Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
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Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

7.4K
Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
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Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

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Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
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相关实验视频

Updated: May 30, 2025

Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy oSLO and Optical Coherence Tomography OCT
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Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy oSLO and Optical Coherence Tomography OCT

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扫描源可见光光学连贯性断层扫描,可见光光学连贯性断层扫描

Weijia Fan, Roman Kuranov, David A Miller

    Optics letters
    |January 31, 2025
    PubMed
    概括
    此摘要是机器生成的。

    我们通过转换近红外激光器开发了扫描可见光源光学相干断层扫描 (SS-vs-OCT). 这种新的SS-vs-OCT成像为生物组织可视化提供了更深的深度和更好的性能.

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    Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy oSLO and Optical Coherence Tomography OCT
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    科学领域:

    • 生物医学光学 生物医学光学
    • 光学成像技术的成像
    • 激光技术 激光技术 激光技术

    背景情况:

    • 可见光光学连贯性断层扫描 (vis-OCT) 为高分辨率成像提供了潜力.
    • 现有的光谱域对OCT系统在成像深度和信号滚动方面存在局限性.

    研究的目的:

    • 为了证明扫描源可见光光学连贯性断层扫描 (SS-vs-OCT) 的可行性.
    • 与光谱域对OCT相比,改进成像深度和信号滚动.

    主要方法:

    • 采用了用于第二和生成 (SHG) 的周期极化酸 (PPLN) 晶体.
    • 将近红外扫描源 (NIR-SS) 激光器转换为可见光SS激光器,通过助推光学放大器 (BOA) 放大,以实现580μW功率.
    • 使用3D打印的金字塔幻影验证性能,通过扫描电子显微镜 (SEM) 进行交叉验证的测量.

    主要成果:

    • 在空气中达到7.3微米 (5.4微米在组织中) 的最大轴分辨率.
    • 在空气中达到5毫米的成像深度 (在组织中达到3.7毫米).
    • 与光谱域对OCT相比,成像深度增加了2.2倍,滚动率提高了2.8倍.

    结论:

    • 扫描可见光源OCT (SS-vs-OCT) 是可行的,并且比现有方法提供了显著的优势.
    • 开发的SS-vis-OCT系统为高分辨率可视化提供了增强的成像深度和更好的性能.
    • 这项技术对先进的生物医学成像应用具有前景.