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Updated: May 30, 2025

2D-HELS MS Seq: A General LC-MS-Based Method for Direct and de novo Sequencing of RNA Mixtures with Different Nucleotide Modifications
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2D-HELS MS Seq: A General LC-MS-Based Method for Direct and de novo Sequencing of RNA Mixtures with Different Nucleotide Modifications

Published on: July 10, 2020

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链切割脱氧酶使mRNA的中下序列通过质谱学实现,没有前端分离

Sarah Mutchek1, Thomas Kenderdine1, Kevin Turner2

  • 1Department of Chemistry, University of Connecticut, Storrs, Connecticut 06269, United States.

Analytical chemistry
|January 31, 2025
PubMed
概括

切割RNA的脱氧化酶,如依赖histidine的脱氧化酶3 (HD3),精确切割大RNA分子用于质谱 (MS) 分析. 这种方法简化了复杂的混合物,使全面的RNA表征成为可能.

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