Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

13.0K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
13.0K
Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

4.6K
Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
4.6K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Fast Multi-Dimensional Imaging Using the Unsupervised 3D Noise2Void Denoising Network.

Analytical chemistry·2025
Same author

Label-free mitochondrial dynamics analysis during cell death using organelle-specific phase contrast microscopy (OS-PCM).

Biomedical optics express·2025
Same author

Real-Time Analysis of Lipid Droplet Morpho-Chemical Dynamics in Living Human Hepatocytes via Phase-Guided Raman Sampling.

Analytical chemistry·2024
Same author

Deep learning-based size prediction for optical trapped nanoparticles and extracellular vesicles from limited bandwidth camera detection.

Biomedical optics express·2024
Same author

Organelle-specific phase contrast microscopy (OS-PCM) enables facile correlation study of organelles and proteins.

Biomedical optics express·2024
Same author

Highly Sensitive, Portable Detection System for Multiplex Chemiluminescence Analysis.

Analytical chemistry·2023
Same journal

Multi-module collaborative optimization-driven fast speckle correlation imaging in variable environments.

Journal of the Optical Society of America. A, Optics, image science, and vision·2026
Same journal

Secrecy performance analysis of NOMA-UWOC systems over a vertically stratified WGG oceanic turbulence channel.

Journal of the Optical Society of America. A, Optics, image science, and vision·2026
Same journal

Backscattering of plane waves in a composite system containing a rough surface and anisotropic scatterers.

Journal of the Optical Society of America. A, Optics, image science, and vision·2026
Same journal

Aspherical surface construction methods based on extended Jacobi polynomials.

Journal of the Optical Society of America. A, Optics, image science, and vision·2026
Same journal

OCT sidelobe suppression method based on dual-path phase sinusoidal modulation and minimum value fusion.

Journal of the Optical Society of America. A, Optics, image science, and vision·2026
Same journal

Optical design concepts using wavelength-selective diffractive optics to enable miniaturized multimodal endoscopic imaging across separated spectral ranges.

Journal of the Optical Society of America. A, Optics, image science, and vision·2026
查看所有相关文章

相关实验视频

Updated: May 30, 2025

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors
11:15

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors

Published on: May 30, 2016

25.1K

内部聚焦相光学转移功能用于空间光干扰显微镜.

Jingde Fang, Peng Liu, Zachary J Smith

    Journal of the Optical Society of America. A, Optics, image science, and vision
    |January 31, 2025
    PubMed
    概括
    此摘要是机器生成的。

    我们开发了一种名为iPOTF的新相光转移函数 (POTF),它假设对象的焦点位于样本内部. 这个iPOTF可以改善图像对比度,并减少高分辨率相位成像中的工件.

    更多相关视频

    Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy oSLO and Optical Coherence Tomography OCT
    12:22

    Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy oSLO and Optical Coherence Tomography OCT

    Published on: August 4, 2018

    8.5K
    High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip
    14:09

    High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip

    Published on: November 16, 2019

    6.8K

    相关实验视频

    Last Updated: May 30, 2025

    A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors
    11:15

    A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors

    Published on: May 30, 2016

    25.1K
    Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy oSLO and Optical Coherence Tomography OCT
    12:22

    Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy oSLO and Optical Coherence Tomography OCT

    Published on: August 4, 2018

    8.5K
    High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip
    14:09

    High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip

    Published on: November 16, 2019

    6.8K

    科学领域:

    • 光学和光子学 在光学和光子学.
    • 显微镜成像技术 显微镜成像技术
    • 图像处理 图像处理

    背景情况:

    • 相光转移功能 (POTF) 对于高分辨率相位成像至关重要.
    • 现有的POTF模型与实验数据不一致,并且在解卷过程中产生有限的对比度改善与持久的光环工件.

    研究的目的:

    • 导出一个新的POTF模型,准确地反映相位成像中的实验观测.
    • 通过减少工件和改善对比度来增强图像解卷.

    主要方法:

    • 通过假设目标镜头在样本平面内聚焦,衍生出一种新的POTF,称为样本内POTF (iPOTF).
    • 将iPOTF模型应用于图像解卷处理过程.

    主要成果:

    • 与传统的POTF相比,新的iPOTF模型与实验结果更接近.
    • 使用iPOTF的解卷显著提高了微弱结构的对比度.
    • iPOTF有效地减少光环工件,优于传统的POTF.

    结论:

    • 在样本中假设客观焦点对于准确的POTF建模至关重要.
    • 在高分辨率相位成像中,iPOTF提供了一种优越的去卷积方法,从而获得更清晰,更详细的图像.