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突菌和Citrobacter freundii之间的合作机制,增强了生物矿物化的机制

Qian Cai¹, Wandong Zhao², Jinping Wang²

¹College of Environmental Science, Sichuan Agricultural University, Chengdu 611130, China; Centro de Biología Molecular Severo Ochoa (CBMSO), Nicolás Cabrera 1, Madrid 28049, Spain.

Journal of hazardous materials

|January 31, 2025

概括

一种Bacillus thuringiensis (BT) 和Citrobacter freundii (CF) 细菌的合作系统通过微生物诱导碳酸盐沉 (MICP) 显著增强了的去除,达到97.68%的效率. 这种协同方法为重金属整治提供了一个新的视角.

科学领域:

环境微生物学环境微生物学
生物修复技术生物修复技术
地质化学地质化学

背景情况:

尿溶性细菌的活力对于修复中的微生物诱导碳酸盐沉 (MICP) 是至关重要的.
在当前的MICP重金属去除应用中,单个细菌的性能限制了效率.

研究的目的:

通过使用MICP,研究Bacillus thuringiensis (BT) 和Citrobacter freundii (CF) 合作系统在 (Cd2+) 固定化方面的协同机制.
通过克服单个尿溶性细菌菌株的局限性,提高Cd2+去除的效率.

主要方法:

从土壤中分离和描述BT和CF菌株.
构建一个BT+CF合作系统 (1:1,v/v) 和接触Cd2+.
代谢分析以确定尿素上调的途径.
使用SEM-EDS,TEM,XPS,XRD和FTIR进行表征,以探索固定机制.

主要成果:

BT+CF合作系统实现了97.68%的Cd2+去除,明显高于单个BT (66.66%) 和CF (88.61%).
代谢分析显示,胺代谢,尿素循环和氨酸降解是尿素上调的关键途径.
MICP过程形成了稳定的碳酸多晶体 (瓦特里特和石),通过各种表征技术证实了这一点.

结论:

在BT+CF合作系统中,通过尿溶性细菌中的协同分子机制,证明了有效的Cd2+固定.
这种协同方法为应用MICP技术在重金属修复中提供了一种新且有效的战略.
了解细菌合作可以提高MICP在环境应用中的潜力.

关键词:

B. thuringiensisensis 这种植物是 C. 的朋友们.合作系统合作系统代谢学代谢学代谢学微生物诱导的碳酸盐沉

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