开发一种快速的X射线光方法,用于在大豆粒中确定蛋白质
Rachel Ferraz de Camargo1, Tiago Rodrigues Tavares1, Felipe Rodrigues Dos Santos1
1Laboratory of Nuclear Instrumentation (LIN), Center for Nuclear Energy in Agriculture (CENA), University of São Paulo (USP), Piracicaba, São Paulo 13416000, Brazil.
Food chemistry
|January 31, 2025
概括
通过分析硫,X射线光 (XRF) 可以准确地测量大豆蛋白质含量. 部分最小平方回归 (PLS) 显示出最佳性能,为现场监测提供了一种可行的方法.
科学领域:
- 农业科学 农业科学
- 分析化学 分析化学
- 频谱学是一种光谱学.
背景情况:
- 要素分析对于确定作物营养价值至关重要.
- X射线光 (XRF) 是一种强大的元素测定技术.
- 准确量化大豆中的蛋白质对于农业和工业应用至关重要.
研究的目的:
- 评估X射线光 (XRF) 的有效性,以量化大豆蛋白质含量.
- 为了比较不同的回归方法 (单变线性回归,多变线性回归,PLS) 用于蛋白质预测.
- 评估使用XRF实时监测大豆蛋白的可行性.
主要方法:
- 使用X射线光 (XRF) 谱法对大豆样本进行基本分析.
- 校准和验证使用两个场景 (A和B) 进行,采用不同的样本集和蛋白质含量范围.
- 部分最小平方 (PLS) 回归被确定为数据分析的主要化学测量方法.
主要成果:
- 部分最小平方 (PLS) 回归在验证中表现出优异的性能,达到0.73 (情景A) 和0.89 (情景B) 的R2值.
- 开发的基于XRF的方法在大豆蛋白质量化方面实现了低于3.1%的相对预测误差.
- 该研究表明,XRF检测到的硫排放与大豆蛋白质含量之间存在很强的相关性.
结论:
- X射线光 (XRF) 光谱,特别是与PLS回归相结合时,提供了一种可靠的方法来量化大豆蛋白质含量.
- 开发的技术为监测大豆中的蛋白质含量提供了一个实用和高效的替代方案.
- 这些发现支持将XRF传感器集成到农业机械中,例如收割机,用于立即选应用.
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