类型Vl分泌系统的OmpR介导激活驱动了Cronobacter中增强的酸耐受性
Yang Wang1, Rui Jiao2, Xiyan Zhang2
1College of Biological and Food Engineering, Anhui Polytechnic University, 241000 Wuhu, China; School of Food and Biological Engineering, Hefei University of Technology, 230601 Hefei, China.
Journal of dairy science
|January 31, 2025
概括
响应调节器OmpR对于Cronobacter来说至关重要.
科学领域:
- 微生物学 微生物学
- 食品安全 食品安全
- 细菌病原体的产生
背景情况:
- 克罗诺细菌是重要的食源性病原体,以其在酸性条件下生存的能力而闻名.
- 耐酸性是Cronobacter在食物和宿主环境中持久性的关键.
- 克罗诺细菌酸性适应的基础分子机制需要进一步阐明.
研究的目的:
- 为了研究两组系统响应调节器OmpR在克罗诺巴克特酸耐受性中的作用.
- 确定OmpR,VI型分泌系统 (T6SS) 和酸适应之间的关系.
- 评估这种OmpR-T6SS介导的酸耐受机制在克罗诺细菌物种中的流行程度.
主要方法:
- 在Cronobacter malonaticus中,在酸性压力下对 ompR 和 T6SS 基因的基因表达分析.
- 在酸性条件下对OmpR和T6SS突变体的构造和生存测定.
- 促进体结合测试以确认OmpR与T6SS促进体的相互作用.
- 对6种额外的Cronobacter物种中ompR淘汰突变的比较分析.
主要成果:
- 酸性压力显著上调了C. malonaticus的OmpR和T6SS基因表达.
- 在酸性压力下OmpR和T6SS突变体的存活率降低.
- OmpR直接与T6SS促进体结合,增强其表达并促进酸适应和生物膜形成.
- 在其他Cronobacter物种中OmpR缺失也降低了T6SS表达和酸耐受性.
结论:
- 两个组成的系统调节器OmpR在克罗诺细菌的酸性耐受性中起着至关重要的作用.
- OmpR激活了类型VI分泌系统 (T6SS),这对酸适应和生存至关重要.
- 这种OmpR-T6SS调节途径在多种Cronobacter物种中得到保护,这突显了它在病原和食品安全方面的重要性.
相关概念视频
CRISPR and crRNAs
16.6K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
16.6K
GPCRs Regulate Adenylyl Cylase Activity
5.2K
Some GPCRs transmit signals through adenylyl cyclase (AC), a transmembrane enzyme. AC helps synthesize second messenger cyclic adenosine monophosphate (cAMP). AC catalyzes cyclization reaction and converts ATP to cAMP by releasing a pyrophosphate. The pyrophosphate is further hydrolyzed to phosphate by the enzyme pyrophosphatase, which drives cAMP synthesis to completion. However, cAMP is rapidly degraded to 5′ AMP by the enzymes phosphodiesterase (PDE), preventing overstimulation of...
5.2K
Defense Against Bacterial Pathogens
1.4K
The human immune system is a complex network of cells, tissues, and organs that work together to defend the body against bacterial infections. It consists of various immune cells, each playing a specific role in the defense mechanism.
Phagocytes
Phagocytes are the frontline soldiers of the immune system. They include neutrophils and macrophages. Neutrophils are the most abundant type of white blood cell and are quickly mobilized to the site of infection. Macrophages are larger cells that patrol...
Phagocytes
Phagocytes are the frontline soldiers of the immune system. They include neutrophils and macrophages. Neutrophils are the most abundant type of white blood cell and are quickly mobilized to the site of infection. Macrophages are larger cells that patrol...
1.4K
Feedback Inhibition
53.6K
Biochemical reactions are occurring constantly in cells, converting starting substances to different products, usually with the help of enzymes that speed the reactions. Without enzymes, it would take far too long for most reactions to occur to be useful to the cell!
53.6K
Prokaryotic Transcriptional Activators and Repressors
20.8K
The organization of prokaryotic genes in their genome is notably different from that of eukaryotes. Prokaryotic genes are organized, such that the genes for proteins involved in the same biochemical process or function are located together in groups. This group of genes, along with their regulatory elements, are collectively known as an operon. The functional genes in an operon are transcribed together to give a single strand of mRNA known as polycistronic mRNA.
Transcription of prokaryotic...
Transcription of prokaryotic...
20.8K


