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相关概念视频

Microbial Fermentation01:23

Microbial Fermentation

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Fermentation is a crucial anaerobic metabolic process that enables microbes to derive energy from sugar without relying on oxygen or an electron transport chain. This process is fundamental to various biological and industrial applications and is classified based on the metabolic products generated.Role of Pyruvate in FermentationPyruvate and its derivatives serve as key electron acceptors in fermentative pathways. The oxidation of NADH to regenerate NAD+ is essential for the continuation of...
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Bioreactor Design and Operational System01:29

Bioreactor Design and Operational System

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Bioreactors are engineered vessels designed to cultivate microorganisms under controlled conditions for industrial bioprocessing. They maintain sterility and allow precise regulation of pH, temperature, oxygen, and nutrient levels to optimize microbial growth and metabolite production. Bioreactors range from small laboratory units of 1 liter to industrial systems holding up to 500,000 liters, though only about 75% of their volume is actively used for fermentation. The remaining headspace...
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Bioreactor Controls-II01:18

Bioreactor Controls-II

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In aerobic fermentations, oxygen is vital for microbial growth and metabolite production. Since air comprises only about 20% oxygen and the gas is poorly soluble in water—just 9 ppm at 20°C—supplying sufficient oxygen becomes a critical challenge, especially in high-demand processes like yeast growth or citric acid production. Even a fully saturated broth may offer only a few seconds of oxygen availability.To address this, sterile or scrubbed air is introduced into the...
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Batch vs Continuous Culture01:14

Batch vs Continuous Culture

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Fermentation is a foundational biotechnological process used to produce pharmaceuticals, biofuels, enzymes, and food additives. Among industrial strategies, batch and continuous fermentation are the two most widely applied. Although both rely on microbial conversion of substrates into desired products, they differ markedly in operation, productivity, and suitability for specific applications.Batch fermentation occurs in a closed system in which nutrient media and inoculum are added at the...
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Scale-Up Processes01:14

Scale-Up Processes

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The scale-up of microbial fermentation processes is essential in industrial biotechnology, allowing the transition from laboratory-scale experiments to commercial-scale production while aiming to maintain product yield and quality. This process requires meticulous adjustment of equipment design, process parameters, and contamination control strategies to accommodate increasing culture volumes.At the laboratory scale, cultures are typically maintained in 1 to 10-liter glass or autoclavable...
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Upstream Processing01:27

Upstream Processing

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Upstream processing represents a critical phase in biomanufacturing, wherein biological systems such as microorganisms, mammalian cells, or insect cells are cultivated to produce therapeutic proteins, vaccines, enzymes, or other biologically derived products. This phase encompasses all steps from the selection and genetic manipulation of the production organism to the cultivation of cells in bioreactors under tightly controlled environmental conditions.Host Selection and Genetic OptimizationThe...
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相关实验视频

Updated: Apr 11, 2026

Microfluidic Picoliter Bioreactor for Microbial Single-cell Analysis: Fabrication, System Setup, and Operation
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Microfluidic Picoliter Bioreactor for Microbial Single-cell Analysis: Fabrication, System Setup, and Operation

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流式发酵:用于全细胞生物生产过程的微系统.

Lina Hollmann1, Lars M Blank2, Alexander Grünberger1

  • 1Institute of Process Engineering in Life Sciences, Microsystems in Bioprocess Engineering (MBVT), Karlsruhe Institute of Technology (KIT), Fritz-Haber-Weg 2, 76131 Karlsruhe, Germany.

Trends in biotechnology
|January 31, 2025
PubMed
概括
此摘要是机器生成的。

微系统为全细胞生物生产提供了一种新的方法,使高效,可扩展的制造成为可能. 这种称为流体发酵的技术为工业生物技术应用开辟了新的途径.

关键词:
生物处理生物处理.生物生产生物制造连续的生物制造.流动生物催化剂的生物催化剂微流体学 在微流体学方面微系统微观系统

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相关实验视频

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Generic Protocol for Optimization of Heterologous Protein Production Using Automated Microbioreactor Technology
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科学领域:

  • 工业生物技术 工业生物技术
  • 生物工艺工程 生物工艺工程
  • 微流体学 微流体学

背景情况:

  • 工业生物技术依赖于大型生物反应器,以使用整个细胞生产有价值的化合物.
  • 生物反应器微型化已经提升了生物过程的理解和优化.
  • 微系统已在化学和生物催化学中建立,但在全细胞生物过程中未得到充分利用.

研究的目的:

  • 探索微系统作为全细胞生物生产的生产单位的潜力.
  • 讨论在这个领域应用微系统的基本和转化方面.
  • 引入"流式发酵"作为微尺度生物生产战略.

主要方法:

  • 对微系统特征和优势的审查.
  • 突出目前在微系统中的生产方法.
  • 在微观尺度上构思未来的全细胞生物生产.

主要成果:

  • 微系统具有独特的特性,有利于生物生产.
  • 目前的应用表明可行性,尽管对于全细胞系统来说是有限的.
  • 流式发酵被提议作为一种可扩展的微观生物生产方法.

结论:

  • 微系统对彻底改变全细胞生物生产具有重大前景.
  • 流式发酵为新的生物工艺和应用提供了一条途径.
  • 需要进一步开发才能充分实现微观的全细胞生物生产.