相关实验视频

Updated: May 29, 2025

Antimicrobial Synergy Testing by the Inkjet Printer-assisted Automated Checkerboard Array and the Manual Time-kill Method

Antimicrobial Synergy Testing by the Inkjet Printer-assisted Automated Checkerboard Array and the Manual Time-kill Method

Published on: April 18, 2019

氧硫酸盐与Ce-FcDC作为激活剂的协同抗菌活性

Hui Xie¹, Sihui Xie¹, Li Jiang¹

¹College of Chemistry and Bioengineering, Guilin University of Technology, Guilin, 541006, Guangxi, China.

Mikrochimica acta

|January 31, 2025

概括

Ce-FcDC纳米酶表现出双氧化酶和超氧化物脱酶活性,通过产生氧硫酸盐的活性氧物种,有效抑制细菌生长. 这提供了一种新的抗微生物策略.

科学领域:

材料科学材料科学材料科学
纳米技术纳米技术
生物化学生物化学

背景情况:

纳米酶为各种应用提供可调节的催化性能.
具有联合酶模拟活动的双功能纳米酶非常受欢迎.
控制微生物生长对于医疗保健和环境保护至关重要.

研究的目的:

开发和表征Ce-MOFs与铁基酸联体 (Ce-FcDC) 作为一个双功能纳米酶.
调查Ce-FcDC的过氧化酶 (POD) 模仿和超氧化物脱酶 (SOD) 模仿活动.
为了评估Ce-FcDC和过氧硫酸盐 (PMS) 抑制细菌生长的疗效.

主要方法:

Ce-FcDC纳米酶的合成和表征.
测试以确定POD模仿和SOD模仿活动.
实验评估Ce-FcDC和PMS对大肠杆菌和金黄色杆菌的协同抗菌作用.
采用p-基和异醇酒精的清洁者研究证实了反应性氧物种的产生.

主要成果:

Ce-FcDC表现出显著的POD模仿活性,催化PMS水解以产生SO4•−,OH和H2O2.
Ce-FcDC展示了SOD模仿活动,将O2•−转化为H2O2.2.
Ce-FcDC和PMS的组合协同抑制了大肠杆菌和金黄色杆菌的生长,比单独使用的更有效.
包括SO4•−,O2•−和OH在内的活性氧物种被证实是抑制细菌的关键参与者.

结论:

Ce-FcDC作为一种具有强大的POD和SOD模仿活动的双功能纳米酶.
Ce-FcDC和PMS的协同作用产生了反应性氧物种的尾酒,导致有效的细菌生长抑制.
这种Ce-FcDC/PMS系统为打击微生物感染和污染提供了一个有希望的战略.

关键词:

美国大肠杆菌 (E. coli).黄金色的S. 黄金色的这是Ce-FcDC.过氧化酶模仿活动的活性.

更多相关视频

High-throughput Identification of Synergistic Drug Combinations by the Overlap2 Method

High-throughput Identification of Synergistic Drug Combinations by the Overlap2 Method

Published on: May 21, 2018

Development of a Polymicrobial Colony Biofilm Model to Test Antimicrobials in Cystic Fibrosis

Development of a Polymicrobial Colony Biofilm Model to Test Antimicrobials in Cystic Fibrosis

Published on: September 20, 2024

相关概念视频

Combined Effects of Drugs: Synergism

Combined Effects of Drugs: Synergism

Synergism is a useful mechanism where combining two or more drugs is more effective than each constituent used alone. Such combinations are also called supra-additive interactions. The drugs collectively enhance the final therapeutic effect by acting on different targets. Another advantage is that the low dose of each constituent drug is sufficient to achieve the desired effect. This helps reduce the duration of therapy and lower the adverse effects of these drugs.
Such synergistic combinations...

Surface Membrane Barriers

Surface Membrane Barriers

The skin and mucous membranes serve as the primary line of defense against pathogens by providing both physical and chemical protection. These barriers are essential in preventing the entry and establishment of microbes, thereby maintaining the integrity of the host.
The outer layer of the skin, the epidermis, is a robust barrier comprising layers of closely packed keratinized cells. This dense arrangement prevents microbes from penetrating the body. The periodic shedding of epidermal cells...