Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Viral Mutations00:36

Viral Mutations

32.1K
A mutation is a change in the sequence of bases of DNA or RNA in a genome. Some mutations occur during replication of the genome due to errors made by the polymerase enzymes that replicate DNA or RNA. Unlike DNA polymerase, RNA polymerase is prone to errors because it is not capable of “proofreading” its work. Viruses with RNA-based genomes, like HIV, therefore accrue mutations faster than viruses with DNA-based genomes. Because mutation and recombination provide the raw material...
32.1K
Viral Recombination00:57

Viral Recombination

23.2K
Cells are sometimes infected by more than one virus at once. When two viruses disassemble to expose their genomes for replication in the same cell, similar regions of their genomes can pair together and exchange sequences in a process called recombination. Alternatively, viruses with segmented genomes can swap segments in a process called reassortment.
23.2K
Retroviruses02:33

Retroviruses

12.2K
Retroviruses and retrotransposons both insert copies of their genetic elements into the genome of the host cell. Thus, the viral genes are passed on when the host genome is replicated or translated. A typical retroviral DNA sequence contains 3-4 genes that encode the different proteins required for its structural assembly and function as a molecular parasite. This DNA is transcribed into a single mRNA, which is very similar in structure to conventional mRNAs, i.e., it is capped at the 5’...
12.2K
Retrovirus Life Cycles01:10

Retrovirus Life Cycles

45.6K
Retroviruses have a single-stranded RNA genome that undergoes a special form of replication. Once the retrovirus has entered the host cell, an enzyme called reverse transcriptase synthesizes double-stranded DNA from the retroviral RNA genome. This DNA copy of the genome is then integrated into the host’s genome inside the nucleus via an enzyme called integrase. Consequently, the retroviral genome is transcribed into RNA whenever the host’s genome is transcribed, allowing the...
45.6K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

A distant brown dwarf coplanar to a warm Jupiter and a hot super-Earth.

Nature·2026
Same author

Nomenclature for Tracking of Genetic Variation of Seasonal Influenza Viruses.

Influenza and other respiratory viruses·2026
Same author

Pre-existing neutralizing antibodies against cattle-transmitted influenza A virus H5N1 are detectable in unexposed individuals.

Immunity·2026
Same author

T cell epitope mapping reveals immunodominance of evolutionarily conserved regions within SARS-CoV-2 proteome.

iScience·2025
Same author

T cell epitope mapping reveals immunodominance of evolutionarily conserved regions within SARS-CoV-2 proteome.

bioRxiv : the preprint server for biology·2024
Same author

Energy landscapes of peptide-MHC binding.

PLoS computational biology·2024
Same journal

Isolation of Mesenchymal Stem Cell-Derived Extracellular Vesicles.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Modeling Melanoma Immune Surveillance by CAR-T Cells in Human Skin Organoids.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Stepwise Optimization of a Matrigel-Based In Vitro Angiogenesis Assay for Reproducible and Quantifiable 2D-Tube Formation Using HUVECs.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Quantifying Mechanical Properties of Fresh Ovarian Tissue with Optical Brillouin Microscopy.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

3D Chromatin Architecture During Early Development: New Methods and New Findings.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Metabolic Plasticity in Embryogenesis Throughout the Lens of NAD<sup></sup>.

Methods in molecular biology (Clifton, N.J.)·2026
查看所有相关文章

相关实验视频

Updated: May 29, 2025

Reverse Genetics to Engineer Positive-Sense RNA Virus Variants
15:49

Reverse Genetics to Engineer Positive-Sense RNA Virus Variants

Published on: June 9, 2022

1.2K

预测病毒演变的概念和方法

Matthijs Meijers1, Denis Ruchnewitz1, Jan Eberhardt1

  • 1Institute for Biological Physics, University of Cologne, Köln, Germany.

Methods in molecular biology (Clifton, N.J.)
|January 31, 2025
PubMed
概括
此摘要是机器生成的。

预测季节性流感病毒的演变对于疫苗开发至关重要. 这项研究提出了一个数据驱动的管道,整合了各种数据,以准确地预测病毒演变和疫苗菌株选择.

关键词:
抗原进化 抗原进化健身模型 健身模型接种流感疫苗可以预防流感人口免疫力的人口免疫力.

更多相关视频

Isolation of Fidelity Variants of RNA Viruses and Characterization of Virus Mutation Frequency
18:10

Isolation of Fidelity Variants of RNA Viruses and Characterization of Virus Mutation Frequency

Published on: June 16, 2011

29.5K
Prediction of HIV-1 Coreceptor Usage Tropism by Sequence Analysis using a Genotypic Approach
07:06

Prediction of HIV-1 Coreceptor Usage Tropism by Sequence Analysis using a Genotypic Approach

Published on: December 1, 2011

13.1K

相关实验视频

Last Updated: May 29, 2025

Reverse Genetics to Engineer Positive-Sense RNA Virus Variants
15:49

Reverse Genetics to Engineer Positive-Sense RNA Virus Variants

Published on: June 9, 2022

1.2K
Isolation of Fidelity Variants of RNA Viruses and Characterization of Virus Mutation Frequency
18:10

Isolation of Fidelity Variants of RNA Viruses and Characterization of Virus Mutation Frequency

Published on: June 16, 2011

29.5K
Prediction of HIV-1 Coreceptor Usage Tropism by Sequence Analysis using a Genotypic Approach
07:06

Prediction of HIV-1 Coreceptor Usage Tropism by Sequence Analysis using a Genotypic Approach

Published on: December 1, 2011

13.1K

科学领域:

  • 病毒学和流行病学.
  • 计算生物学和生物信息学
  • 免疫学和疫苗开发

背景情况:

  • 季节性人类流感病毒由于适应性突变而迅速演变,主要是在抗体准的血凝素表位上.
  • 流通病毒株的年度变化需要持续监测和预测,以进行有效的公共卫生干预.
  • 目前用于预测病毒演变和选择疫苗菌株的方法可以通过综合数据分析来增强.

研究的目的:

  • 描述一个一致的,数据驱动的方法论,用于季节性人类流感病毒演变的预测分析.
  • 为了能够准确预测流通的病毒菌株,并告知预防性疫苗菌株选择.
  • 为预测候选疫苗菌株对未来病毒种群的疗效提供一个框架.

主要方法:

  • 整合了四种关键数据类型:全球病毒分离序列数据,流行病学发病率数据,抗原特征和内在病毒表型.
  • 开发一个用于分析这些集成数据集的计算管道.
  • 估计相对适合循环菌株的相对适应性,并预测高达一年的类频率.

主要成果:

  • 该管道成功估计了流感菌株的相对适应性,并预测了未来的类频率.
  • 对疫苗菌株对预测未来病毒种群的保护进行比较估计.
  • 该方法为数据驱动的预防性疫苗菌株选择提供了基础.

结论:

  • 一个强大的,数据驱动的管道可以准确地预测季节性流感病毒的演变,并告知疫苗菌株选择.
  • 整合多种数据源可以提高病毒演变和疫苗有效性的预测能力.
  • 这种方法支持积极的公共卫生战略,以对抗快速演变的流感病毒和SARS-CoV-2.