相关实验视频

Updated: May 2, 2026

Intra-tracheal Administration of Haemophilus influenzae in Mouse Models to Study Airway Inflammation

Intra-tracheal Administration of Haemophilus influenzae in Mouse Models to Study Airway Inflammation

Published on: March 2, 2016

皮埃佐1指向的中性粒细胞外陷在流感病毒感染期间调节巨细胞分化

Yuexin Wang¹, Qiuli Yang¹, Yingjie Dong¹

¹Key Laboratory of Cell Proliferation and Regulation Biology, Ministry of Education, College of Life Sciences, Beijing Normal University, 100875, Beijing, China.

Cell death & disease

|January 31, 2025

概括

离子通道Piezo1在流感病毒感染期间调节免疫细胞通信. Piezo1控制中性粒细胞外细胞陷 (NETs) 和巨细胞分化,影响抗病毒免疫力.

科学领域:

免疫学免疫学免疫学
细胞生物学细胞生物学
病毒学病毒学

背景情况:

中性粒细胞和巨细胞对于抗病毒免疫力至关重要.
它们在病毒感染期间的精确调节相互作用尚未完全理解.

研究的目的:

调查Piezo1离子通道在抗流感病毒免疫期间调节中性粒细胞和巨细胞功能的作用.
阐明Piezo1影响免疫细胞交叉声和巨细胞谱系承诺的机制.

主要方法:

在中性粒细胞中利用了Piezo1的遗传删除.
研究了中性粒细胞外细胞陷 (NET) 的形成和巨细胞分化在体外和体内.
分析了信号通路,包括TLR9,cGAS-STING,信号,以及SIRT2-HIF1α通路.

主要成果:

中性粒细胞中Piezo1的遗传删除损害了NET形成和M1巨分化,促进了M2巨的发展.
来自中性粒细胞的NET DNA被确定为巨细胞分化的直接调节者.
皮埃佐1缺乏导致TLR9和cGAS-STING信号减少,并使巨细胞偏向从M1到M2.

结论:

Piezo1 是中性粒细胞外细胞陷形成和巨细胞在抗病毒免疫中的分化的一个关键调节器.
由Piezo1调节的中性粒细胞衍生的NET DNA在指导巨细胞谱系承诺方面发挥着关键作用.
Piezo1集成信号传递和SIRT2-HIF1α通路,在流感感染期间协调巨细胞两极分化.

更多相关视频

Visualizing Macrophage Extracellular Traps Using Confocal Microscopy

Visualizing Macrophage Extracellular Traps Using Confocal Microscopy

Published on: October 19, 2017

Imaging Cell Interaction in Tracheal Mucosa During Influenza Virus Infection Using Two-photon Intravital Microscopy

Imaging Cell Interaction in Tracheal Mucosa During Influenza Virus Infection Using Two-photon Intravital Microscopy

Published on: August 17, 2018

相关概念视频

Cell-mediated Immune Responses

Cell-mediated Immune Responses

Cell Motility through Blebbing

Cell Motility through Blebbing

Blebs are a type of membrane protrusion formed by the internal hydrostatic pressure of the cytoplasm. Blebs are observed in several cell types, including fibroblasts, immune cells, and single-celled organisms like the amoeba. The primary function of blebs is cell locomotion and apoptosis, but they are also found during necrosis and cell division. The life cycle of a bleb comprises an initiation phase followed by the expansion and retraction phases.
Blebbing Through the Matrix
In multicellular...

Differentiation of Common Myeloid Progenitor Cells

Differentiation of Common Myeloid Progenitor Cells

Common myeloid progenitors (CMPs) are oligopotent cells that can differentiate into granulocytes and macrophages. Granulocytes and macrophages are essential for protecting the body against bacterial, viral, or fungal infections. They migrate from the bone marrow into the circulating blood to reach specific tissue sites where they differentiate and help in immune surveillance. However, they survive only for a few days and must be continuously made available to the organism to maintain a robust...

B Cell Activation and Differentiation

B Cell Activation and Differentiation

The adaptive immune response, a sophisticated defense mechanism, relies on the activation and differentiation of B lymphocytes, or B cells. These processes enable our bodies to mount a tailored response against specific pathogens such as bacteria, free virus particles, toxins, and parasites.
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...