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氧化种子原料增强小麦幼苗的干旱耐受性,通过改善抗氧化活性和保护

Rasha M El-Shazoly¹, A A Othman², Muhammad Saqlain Zaheer³

¹Botany and Microbiology Department, Faculty of Science, New Valley University, Al-Kharja, New Valley, 72511, Egypt. dr.rasha_elshazly@sci.nvu.edu.eg.

Scientific reports

|January 31, 2025

概括

用氧化纳米颗粒 (ZnO NPs) 或散装氧化 (ZnO) 进行种子原料化,有效抵消小麦幼苗的干旱压力. 这种治疗增强了抗氧化剂防御,并改善了生理参数,这对于植物在缺水的情况下生存至关重要.

科学领域:

植物生理学植物生理学
农业科学农业科学
纳米技术在农业中的应用

背景情况:

干旱压力严重影响植物生长和种子发芽,导致脱水和破坏生物分子功能.
对于植物的新陈代谢,细胞完整性和抗压力至关重要,使其成为减轻干旱影响的潜在剂.
种子初始化是一种用于提高种子性能和耐压力的技术.

研究的目的:

研究氧化纳米颗粒 (ZnO NPs) 和散装ZnO种子原料在减轻小麦 (Triticum aestivum L.) 幼苗的干旱压力的有效性.
为了评估在ZnO种子原始化后,在干旱压力下小麦幼苗的生理和代谢反应.

主要方法:

进行了一项盆栽实验,使用受不同干旱程度 (80%和60%的田间容量) 的小麦苗.
种子用ZnO NPs (纳米原料) 或散装ZnO (60毫克L-1) 进行了原料化.
测量了包括抗氧化酶活性 (POD),含量,H2O2清除,脂质过氧化抑制,自由氨基酸和可溶碳水化合物在内的生理参数. 实验和计算方法 (TD-DFT) 用于对ZnO NPs/Iso分子的IR和XRD分析.

主要成果:

用ZnO NPs或散装ZnO进行种子原料处理显著减轻了干旱压力的负面影响,特别是在60%的田间容量下.
原始化治疗导致POD活性,含量,H2O2吸收和脂质过氧化抑制在芽和根的实质性增加.
与未经培养的对照组相比,在初级化,干旱压力下的幼苗中,观察到自由氨基酸和可溶碳水化合物含量的显著改善.

结论:

用ZnO NPs或散装ZnO进行种子原料化提供了有效的机械和生理保护,防止小麦苗木遭受干旱压力.
观察到的改善归因于增强的抗氧化酶活性,增加的非酶成分和升高的护剂水平.
在干旱和半干旱环境中,ZnO种子原料化是一种有前途的策略,可以提高作物弹性.

关键词:

在Triticum aestivum中,它是最重要的.抗氧化剂系统抗氧化剂系统干旱造成的压力是干旱.自由基的清理能力.纳米原料制造 (nanopriming) 是一种技术.在TD-DFT计算过程中.ZnO NPs的使用情况.有氧化 (ZnO) 的散装.

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