生物处理模式的可转移性用于重组蛋白酶的生产:从食批量到用Bacillus licheniformis进行连续培养
Stefan Kittler1, Fabian Müller1, Mohamed Elshazly1
1Research Division Integrated Bioprocess Development, Institute of Chemical, Environmental and Bioscience Engineering, TU Wien, Gumpendorfer Strasse 1a, Vienna, 1060, Austria.
BMC biotechnology
|January 31, 2025
概括
持续培养Bacillus licheniformis蛋白酶为养批量方法提供了一个可持续的替代方案. 虽然食批量产量更高,但连续方法为优化工业酶生产提供了宝贵的见解.
科学领域:
- 生物技术是生物技术.
- 工业微生物学 工业微生物学
背景情况:
- 蛋白酶是重要的工业酶,由于其快速生长和细胞外分泌,Bacillus菌株是重组生产的理想选择.
- 传统的批量和料批量发酵是能源密集的,并且有很长的停机时间,这引起了人们对持续种植的兴趣.
- 有限的研究存在于连续培养的细菌菌株,需要探索这种方法.
研究的目的:
- 通过无压缩感应系统来研究化学定位培养,以生产Bacillus licheniformis蛋白酶.
- 为了比较连续种植的特定生产率和时空产量与食批量方法.
- 建立连续种植作为工业蛋白质酶生产中料批次的竞争性替代品.
主要方法:
- 利用化学定位培养用于Bacillus licheniformis蛋白酶生产,使用无压缩诱导系统.
- 采用混合模型来描述连续种植,分析生产率作为稀释率和生成时间的函数.
- 将特定生产率和时空产量与已建立的料批种植技术进行比较.
主要成果:
- 在同一个增长率的料批和化学定位模式下进行的选,为可减压系统带来了不同的特定生产率.
- 食率对特定生产率的影响在食批量和化学定位种植之间有显著差异.
- 稳定的连续运作实现了三分之一的空间时间收益率在料批量种植中观察到.
结论:
- 食批量查可能不足以开发Bacillus licheniformis.中去压促销体系统的连续过程.
- 连续种植,虽然不超过料批次的时空产量,但为流程优化提供了宝贵的机械洞察力.
- 这项研究有助于为工业化蛋白质酶生产转向更可持续的种植方法.
相关概念视频
01:29Bioreactor Design and Operational System
Bioreactors are engineered vessels designed to cultivate microorganisms under controlled conditions for industrial bioprocessing. They maintain sterility and allow precise regulation of pH, temperature, oxygen, and nutrient levels to optimize microbial growth and metabolite production. Bioreactors range from small laboratory units of 1 liter to industrial systems holding up to 500,000 liters, though only about 75% of their volume is actively used for fermentation. The remaining headspace...
01:14Batch vs Continuous Culture
Fermentation is a foundational biotechnological process used to produce pharmaceuticals, biofuels, enzymes, and food additives. Among industrial strategies, batch and continuous fermentation are the two most widely applied. Although both rely on microbial conversion of substrates into desired products, they differ markedly in operation, productivity, and suitability for specific applications.Batch fermentation occurs in a closed system in which nutrient media and inoculum are added at the...
01:23Fed-Batch Culture
Fed-batch culture is a widely used bioprocessing strategy combining aspects of batch culture with controlled substrate feeding to optimize cell growth and product formation. In this semi-closed system, nutrients are strategically added during fermentation, while the accumulated products and biomass remain within the bioreactor until the end of the operation. This controlled addition of substrates allows for better management of growth kinetics, nutrient limitation, and metabolite...
01:14Scale-Up Processes
The scale-up of microbial fermentation processes is essential in industrial biotechnology, allowing the transition from laboratory-scale experiments to commercial-scale production while aiming to maintain product yield and quality. This process requires meticulous adjustment of equipment design, process parameters, and contamination control strategies to accommodate increasing culture volumes.At the laboratory scale, cultures are typically maintained in 1 to 10-liter glass or autoclavable...
01:27Upstream Processing
Upstream processing represents a critical phase in biomanufacturing, wherein biological systems such as microorganisms, mammalian cells, or insect cells are cultivated to produce therapeutic proteins, vaccines, enzymes, or other biologically derived products. This phase encompasses all steps from the selection and genetic manipulation of the production organism to the cultivation of cells in bioreactors under tightly controlled environmental conditions.Host Selection and Genetic OptimizationThe...
01:27Production of Antibiotics
Penicillin, one of the earliest and most widely used antibiotics, is produced industrially by the filamentous fungus Penicillium chrysogenum. Large stirred-tank bioreactors ranging from tens to hundreds of thousands of liters maintain tightly controlled temperature, pH, and dissolved oxygen conditions to support fungal metabolism and maximize antibiotic yield. Penicillin is a secondary metabolite, synthesized primarily during the stationary growth phase, which requires a carefully managed...