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Updated: May 29, 2025

Characterization of Immune Cell-derived Extracellular Vesicles and Studying Functional Impact on Cell Environment

Characterization of Immune Cell-derived Extracellular Vesicles and Studying Functional Impact on Cell Environment

Published on: June 2, 2020

来自Streptococcus thermophilus 065的细胞外囊泡的特征及其调节免疫反应的潜力

Angela Rocio Ortiz Camargo¹, Oscar van Mastrigt¹, Joost W Gouw²

¹Food Microbiology, Wageningen University & Research, PO Box 17, 6700 AA, Wageningen, The Netherlands.

Probiotics and antimicrobial proteins

|February 1, 2025

概括

热球菌 (Streptococcus thermophilus) 释放细胞外囊泡 (EVs),携带各种蛋白质,包括菌体蛋白质. 这些细菌EV调节免疫细胞的反应,表明后生物的潜力.

科学领域:

微生物学微生物学
免疫学免疫学免疫学
生物化学生物化学

背景情况:

细菌释放细胞外囊泡 (EVs) 进行细胞间通信.
热菌 (Streptococcus thermophilus EVs) 参与微生物与微生物和微生物与宿主之间的相互作用.

研究的目的:

为了调查Streptococcus thermophilus 065.5的EV生产情况.
分析这些EV的蛋白质组成.
为了确定EVs在体外对人类免疫细胞的影响.

主要方法:

在特定条件下培养S. thermophilus以最大限度地提高EV产量.
使用脂友的光染料染色量化EVs.
通过质谱学对孤立的EV进行蛋白质组分析.
在体外免疫试验中使用人类外周血液单核细胞 (PBMC).

主要成果:

通过在M17乳糖中种植的S. thermophilus实现了高EV产量.
电动汽车富含ABC输送蛋白,核糖体蛋白和糖解酶.
在EV中检测到菌体蛋白质,与菌体基因表达和EV释放有关,进一步受到米托米辛C的诱导.
S. thermophilus EVs对非激活的和激活的PBMCs都表现出免疫调节作用.

结论:

热 EVs是强大的免疫调节器.
菌体蛋白质的存在表明它们在EV生物发生过程中起着作用.
这些EV有望作为后生物在调节免疫反应中的应用.

关键词:

这种菌株是Streptococcus thermophilus.细胞因子 (cytokines) 是一种细胞因子.免疫调节是一种免疫调节.乳酸细菌是一种乳酸细菌.菌体菌体是一种菌体.后生物学的后生物学.蛋白质组学是指蛋白质组学.

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