细菌的西兰酶sp. 在固态发酵下培养的NIORKP76:生产,净化,表征及其糖化潜力
Pankaj D Parab1,2, Rakhee D Khandeparker1
1Biological Oceanography Division, CSIR-National Institute of Oceanography (CSIR-NIO), Dona Paula, Goa, India.
Preparative biochemistry & biotechnology
|February 1, 2025
概括
这项研究优化了来自Bacillus sp.的微生物西兰酶的产生. NIORKP76使用小麦. 该酶有效地将农业残留物转化为生物燃料原料,产生高发酵糖.
科学领域:
- 生物技术是生物技术.
- 酶学 是一种酶学.
- 微生物学 微生物学
背景情况:
- 微生物西兰酶是重要的工业酶.
- 像小麦这样的农业工业废物是酶生产的可持续基质.
- 细菌物种以生产有价值的酶而闻名.
研究的目的:
- 为了优化来自Bacillus sp.的西兰酶生产. NIORKP76 在小麦上使用固态发酵.
- 为了描述产生的西兰酶.
- 评估其在将农业残留物转化为生物燃料原料方面的潜力.
主要方法:
- 在固态状态下发酵Bacillus sp. 在小麦上NIORKP76
- 优化发酵参数 (盐,基质和水分比,时间,温度).
- 酶净化,表征 (pH/温度最佳,稳定性) 和在农业残留物水解中的应用.
主要成果:
- 在最佳条件下,在28°C的温度下,在72小时内产生最大的西兰酶产量.
- 纯化西兰酶 (28 kDa) 在pH值8.0和60°C时表现出最佳活性,稳定性良好.
- 在40°C下用5个单位/克西兰酶对预处理的小麦进行水解,得到了141毫克/克的可发酵糖.
结论:
- 巴西勒斯种类 (Bacillus sp.) 的存在 NIORKP76是工业化西兰酶生产的一个有前途的来源.
- 优化的西兰酶稳定且有效地将小麦转化为生物燃料原料.
- 这一过程为从农业废物中生产生物燃料提供了一个可持续的途径.
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