酸受体在5亿年陆地植物进化过程中功能性地融合在一起
Gil Zimran1, Michal Shpilman1, Eve Hobson2
1The Robert H. Smith Institute of Plant Sciences and Genetics in Agriculture, the Hebrew University of Jerusalem, 7610000 Rehovot, Israel.
Current biology : CB
|February 1, 2025
概括
进化了独特的酸 (ABA) 受体,其基底活性较低,减轻了祖先的高信号传递. 这种独立的进化路径增强了植物适应的ABA信号幅度.
科学领域:
- 植物生物学 植物生物学
- 分子进化分子进化
- 生物化学 生物化学
背景情况:
- 酸 (ABA) 对于植物脱水反应和陆地适应是至关重要的.
- 核心ABA信号模块涉及抑制2C型蛋白酸酶 (PP2Cs) 的PYRABACTIN RESISTANCE 1类蛋白质 (PYLs).
- 祖先的ABA受体具有高的背景信号,限制了动态范围,而血管精子受体进化了二维形式,以严格依赖ABA.
研究的目的:
- 研究中ABA受体的进化起源和特征.
- 确定PYL活动和ABA响应的基础的功能机制.
- 将的ABA受体进化与血管苗的进化进行比较.
主要方法:
- 酵母两杂交测试以评估蛋白质相互作用.
- 试验室PP2C抑制试验测量PYL活性.
- 尺寸排除色谱以确定PYL的寡合状态.
- 突变分析,生化和生理分析以确定功能变异.
主要成果:
- 拥有三个不同的PYL亚家族,基底PP2C抑制活性较低.
- 摩斯PYLs主要是单质的,与血管精子同质体受体不同.
- PYL中的特定氨基酸变异解释了它们的基底活动减少.
- 能够实现二分化的突变显著损害了受体中的ABA响应.
结论:
- 独立进化了基底活性较低的ABA受体,汇聚在一个解决方案,以减轻祖先的高信号.
- 的这种并行进化扩大了ABA信号的动态范围,这对于适应至关重要.
- 单体 () 和二极体 () ABA受体的独特进化轨迹突出了功能约束和适应压力.
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