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停产胚胎中PRMT3基因表达和甲基化水平:对发育停产缺陷的影响

Wuwen Zhang¹, Shifeng Li², Kai Li¹

¹Department of Infertility and Reproductive Medicine, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai, 201203, China.

Developmental biology

|February 1, 2025

概括

胚胎中蛋白质氨酸甲基酶3 (PRMT3) 的降低阻碍了发育. 恢复PRMT3水平部分挽救了被阻止的胚胎,这表明PRMT3对于成功的体外受精 (IVF) 胚胎发育至关重要.

科学领域:

生殖生物学生殖生物学
发育生物学是发展生物学.
分子遗传学分子遗传学

背景情况:

在体外受精 (IVF) 的成功受到早期胚胎发育停止的限制.
蛋白质氨酸甲基酶3 (PRMT3) 在早期发育过程中与基因调节有关,但其确切作用尚不清楚.

研究的目的:

研究PRMT3在胚胎早期发育中的作用及其对试管婴儿结果的潜在影响.
检查停产的试管婴儿胚胎中的PRMT3表达和H4R3me2a甲基化.

主要方法:

使用了IVF的丢弃,停止和多精子胚胎.
使用共聚焦显微镜和qRT-PCR来评估PRMT3表达和H4R3me2a甲基化.
在停止的胚胎中重新表达PRMT3,以评估救援效应.

主要成果:

与对照组相比,被捕胚胎的PRMT3核酸和蛋白质水平显著降低.
在停止的胚胎中,H4R3me2a甲基化水平也显著降低.
PRMT3的重新表达部分挽救了发育停滞,一些胚胎进展到毛囊或胚囊阶段.

结论:

在早期胚胎中,PRMT3的表达或功能减少可能会导致发育停止.
调节PRMT3对于适当的早期胚胎发育至关重要.
对胚胎发育停止的治疗干预措施进行进一步的研究是有必要的.

关键词:

在发展过程中被捕.早期胚胎发育的早期发展.表观遗传调节表观遗传调节在试管婴儿受精体外受精.蛋白质阿尔金氨基甲基酶3 3

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