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Updated: May 29, 2025

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乳制品衍生抗微生物GMp7:破坏蛋白质网络,用于多目标抗菌抑制和增强乳制品保存

Jinze He¹, Hong Li², Yujuan Xu³

¹Sericultural and Agri-Food Research Institute, Guangdong Academy of Agricultural Sciences, Key Laboratory of Functional Foods, Ministry of Agriculture and Rural Affairs, Guangdong Key Laboratory of Agricultural Product Processing, Guangzhou, 510610, China; College of Food Science and Technology, Yunnan Agricultural University, Kunming, 650201, Yunnan, China.

Journal of dairy science

|February 1, 2025

概括

一种新的牛奶,GMp7,通过破坏细菌细胞膜和干扰必需蛋白质,有效地对抗金黄色葡萄球菌. 这种可以提高食品安全,延长乳制品的保质期.

科学领域:

微生物学微生物学
生物化学生物化学
食品科学食品科学食品科学

背景情况:

抗微生物 (AMP) 对于天生的免疫力至关重要,并代表了传统抗生素的有希望的替代品.
黄金葡萄球菌是食品中的重要病原体,需要有效的控制策略.
识别具有强烈抗菌活性的新型AMP对黄金色杆菌具有很高的食品安全意义.

研究的目的:

确定和描述一种新的乳制品衍生抗微生物,GMp7.
为了阐明GMp7的多向抗菌机制对抗黄金葡萄球菌.
评估GMp7在巴氏杀菌牛奶中抑制黄金菌生长的疗效.

主要方法:

福利埃变换红外光谱学,流动细胞计和扫描电子显微镜被用来分析GMp7对细菌细胞的影响.
无标签蛋白质组学分析确定了蛋白质标和量化了由GMp7.7引起的蛋白质组变化.
分子对接被用来预测GMp7与S. aureus关键蛋白质的结合亲和力.

主要成果:

GMp7具有有利于膜破坏的二次结构 (17.45%α螺旋,20.10%β角,37.13%β折).
蛋白质组分析显示,GMp7对99种蛋白质进行下调,对26种蛋白质进行上调,这表明它具有多目标机制.
GMp7强烈结合至关重要的金黄色菌蛋白 asd 和 pcrA,结合能量分别为 -8.477 和 -8.407 kcal/mol.
GMp7 干扰了糖生物合成,ABC 运输,二元系统,DNA 复制和不匹配修复.
GMp7显著抑制了在巴氏消毒牛奶中的金黄色细菌生长.

结论:

GMp7表现出一种强大的,多目标的抗菌机制,用于对抗黄金葡萄球菌.
的破坏细菌膜和抑制必要的细胞过程的能力有助于其疗效.
GMp7在乳制品行业具有很大的应用潜力,可以提高食品安全,延长产品的保质期.

关键词:

中心蛋白质的中心蛋白质.牛奶衍生的酸.保护保护保存保存蛋白质-蛋白质网络蛋白质-蛋白质网络

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