抗体分泌细胞库中含有高亲和度的抗罗库特异性,可以在体内诱导过敏反应
Alice Dejoux1, Qianqian Zhu2, Adam Woolfe3
1Institut Pasteur, Université Paris Cité, Institut National de la Santé et de la Recherche Médicale (INSERM) UMR1222, Antibodies in Therapy and Pathology, Paris, France; Collège Doctoral, Sorbonne Université, Paris, France.
The Journal of allergy and clinical immunology
|February 1, 2025
概括
研究人员确定了特定的抗体,导致肌肉松剂罗库的过敏反应. 这项研究揭示了抗体标,并证明了严重过敏反应的可能性,为神经肌肉阻断剂过敏反应建立了一个新的小鼠模型.
科学领域:
- 免疫学 免疫学 免疫学
- 药理学 药理学是指药理学的学科.
- 结构生物学 结构生物学
背景情况:
- 像罗库这样的神经肌肉阻断剂 (NMBA) 对于机械通风至关重要,但在罕见的情况下可能导致严重的过敏反应.
- 罗库与过敏反应的高率有关,但负责的特定抗体仍未确定.
- 了解罗库诱导过敏的机制对于麻醉中患者的安全至关重要.
研究的目的:
- 从免疫小鼠中识别和表征罗库特异性抗体.
- 确定这些抗体的结合亲缘关系,结构相互作用和诱导过敏反应的潜力.
- 为了阐明罗库触发过敏反应的分子基础.
主要方法:
- 给小鼠免疫了罗库结合蛋白,以产生特定的抗体反应.
- 从排序的血细胞进行了抗体基因 (VH和VL) 的单细胞测序.
- 抗体表达为人类IgG1和IgE,它们的功能活动在体外和体内被评估.
主要成果:
- 确定了两个不同的抗体谱,包括三个主要的克隆家族,具有融合的结合特征.
- 抗体对罗库有很高的亲和力,与相关的NMBA有一定的交叉反应.
- 抗罗库IgE抗体激活了人类的巨细胞和基细胞,并在小鼠模型中诱导了严重的过敏反应.
结论:
- 这项研究确定了针对罗库的抗体所准的特定表位.
- 已经证明了抗罗库IgE抗体的过敏反应潜力.
- 已经建立了一种新的小鼠模型来研究NMBA诱导的过敏反应,这有助于未来的研究和预防策略.
相关概念视频
Cross-reactivity
30.9K
Overview
30.9K
Allergic Reactions
27.2K
Overview
27.2K
Nondepolarizing (Competitive) Neuromuscular Blockers: Mechanism of Action
1.4K
Nondepolarizing neuromuscular blockers induce paralysis by competitively blocking nicotinic acetylcholine receptors at the muscle end plate. Examples include pancuronium, mivacurium, vecuronium, and rocuronium. These quaternary ammonium derivatives are administered intravenously, are poorly absorbed, and are excreted via the kidneys.
Competitive antagonists prevent acetylcholine from binding to its receptor, inhibiting membrane depolarization. Without conformational changes or intrinsic...
Competitive antagonists prevent acetylcholine from binding to its receptor, inhibiting membrane depolarization. Without conformational changes or intrinsic...
1.4K
Nondepolarizing (Competitive) Neuromuscular Blockers: Pharmacological Actions
386
Nondepolarizing neuromuscular blockers prevent the membrane depolarization of muscle cells and inhibit muscle contraction. These are usually administered with anesthetics to achieve complete muscle relaxation. Upon administration, these drugs first block the small, rapidly contracting muscles of the face and hands, followed by the larger muscles of the trunk and the intercostal muscles. The diaphragm is the last muscle to be affected.
Although all competitive neuromuscular blockers are designed...
Although all competitive neuromuscular blockers are designed...
386
Antibody Structure
58.8K
Overview
Antibodies, also known as immunoglobulins (Ig), are essential players of the adaptive immune system. These antigen-binding proteins are produced by B cells and make up 20 percent of the total blood plasma by weight. In mammals, antibodies fall into five different classes, which each elicits a different biological response upon antigen binding.
The Y-Shaped Structure of Antibodies Consists of Four Polypeptide Chains
Antibodies consist of four polypeptide chains: two identical heavy...
Antibodies, also known as immunoglobulins (Ig), are essential players of the adaptive immune system. These antigen-binding proteins are produced by B cells and make up 20 percent of the total blood plasma by weight. In mammals, antibodies fall into five different classes, which each elicits a different biological response upon antigen binding.
The Y-Shaped Structure of Antibodies Consists of Four Polypeptide Chains
Antibodies consist of four polypeptide chains: two identical heavy...
58.8K
Antibody Actions
1.0K
Antibodies, or immunoglobulins, are critical players in the immune system's arsenal against invading pathogens. Produced by B cells and plasma cells, their primary role is to detect and bind to specific antigens, molecules found on the surface of pathogens like bacteria or viruses. Beyond antigen recognition, antibodies perform several vital functions that contribute to immune defense.
Neutralization
Antibodies can bind to pathogens, preventing them from infecting host cells. This process...
Neutralization
Antibodies can bind to pathogens, preventing them from infecting host cells. This process...
1.0K


