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通过结合昼夜节律基因表达和免疫细胞透,探索急性心肌梗塞的潜在生物标志物

Xiao Yu¹, Xiaopeng Zhang¹, Hazrat Bilal¹

¹Department of Pathology and Forensic Medicine, College of Basic Medical Sciences, Dalian Medical University, Dalian, China.

Scientific reports

|February 1, 2025

概括

这项研究确定了五个关键的昼夜节律基因 (CRG),与急性心肌梗塞 (AMI) 病原发生有关. 这些CRG显示出作为AMI的特定诊断生物标志物和治疗点的潜力.

科学领域:

心血管研究研究心血管研究
分子生物学分子生物学
免疫学免疫学免疫学

背景情况:

目前的急性心肌梗塞 (AMI) 生物标志物如热素缺乏特异性,导致错误阳性.
新出现的证据将昼夜节律扰乱和免疫细胞透与AMI发展联系在一起.

研究的目的:

调查与昼夜节律相关的基因 (CRG) 与新型AMI诊断生物标志物的免疫透之间的相互作用.
识别与AMI相关的特定CRG和免疫细胞特征.

主要方法:

在AMI患者和健康对照中对15个CRG的差异基因表达分析.
功能性丰富分析和单个样本基因组丰富分析 (ssGSEA) 用于免疫透.
逆转录定量聚合酶链反应 (RT-qPCR) 在低毒条件下的验证.

主要成果:

在AMI中,五个关键CRG (JUN,NAMPT,S100A8,SERPINA1,VCAN) 被确定为关键.
在AMI患者中观察到高水平的中性粒细胞,巨细胞和氨基粒细胞.
在低氧条件下证实了差异性CRG表达,这表明与AMI病理生理学的联系.

结论:

与昼夜节律相关的基因在急性心肌梗塞中起着重要作用.
已确定的CRG和免疫细胞简介为开发AMI更具体的诊断生物标志物提供了潜力.
这些发现为针对AMI治疗中的CRGs的新型治疗策略铺平了道路.

关键词:

剧烈的心肌梗塞急性心肌梗塞生物信息学分析生物标志物生物标志物与循环节律相关的基因免疫系统的透.

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