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相关概念视频

Conserved Binding Sites01:49

Conserved Binding Sites

4.2K
Many proteins’ biological role depends on their interactions with their ligands, small molecules that bind to specific locations on the protein known as ligand-binding sites. Ligand-binding sites are often conserved among homologous proteins as these sites are critical for protein function.
Binding sites are often located in large pockets, and if their location on a protein’s surface is unknown, it can be predicted using various approaches. The energetic method computationally...
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Conservation of Protein Domains Over Different Proteins02:26

Conservation of Protein Domains Over Different Proteins

10.8K
Protein domains are small structurally independent units that are part of a single amino acid chain.  Although these domains are often structurally independent, they may rely on synergistic effects to perform their functions as part of a larger protein. Protein domains may be conserved within the same organism, as well as across different organisms.
A limited set of protein domains often duplicate and recombine during evolution. These domains can be organized in different combinations to...
10.8K
Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

2.5K
Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order...
2.5K
Ligand Binding and Linkage00:49

Ligand Binding and Linkage

4.7K
Allosteric proteins have more than one ligand binding site; the binding of a ligand to any of these sites influences the binding of ligands to the other sites. When a protein is allosteric, its binding sites are called coupled or linked.  In the case of enzymes, the site that binds to the substrate is known as the active site and the other site is known as the regulatory site. When a ligand binds to the regulatory site, this leads to conformational changes in the protein that can influence...
4.7K
General Transcription Factors01:30

General Transcription Factors

5.2K
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
5.2K
Cooperative Binding of Transcription Regulators02:13

Cooperative Binding of Transcription Regulators

6.3K
Transcriptional regulators bind to specific cis-regulatory sequences in the DNA to regulate gene transcription. These cis-regulatory sequences are very short, usually less than ten nucleotide pairs in length. The short length means that there is a high probability of the exact same sequence randomly occurring throughout the genome.  Since regulators can also bind to groups of similar sequences, this further increases the chances of random binding. Transcriptional regulators form...
6.3K

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相关实验视频

Updated: May 29, 2025

Screening for Functional Non-coding Genetic Variants Using Electrophoretic Mobility Shift Assay EMSA and DNA-affinity Precipitation Assay DAPA
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表观基因的功能多样性:异型的使用,无序的域内容和可变的结合伙伴.

Leroy Bondhus1,2,3, Aileen A Nava1,2,3, Isabelle S Liu1,2,3

  • 1Department of Human Genetics, David Geffen School of Medicine, UCLA, 615 Charles E. Young Drive South, Los Angeles, CA, 90095, USA.

Epigenetics & chromatin
|February 1, 2025
PubMed
概括
此摘要是机器生成的。

对发育和疾病至关重要的表原体表现出独特的结构特征,如更大的尺寸,更多的外因子和内在无序的区域,从而实现了多样化的功能. 这些发现有助于开发针对表观基因相关疾病和癌症的向疗法.

关键词:
染色体修饰剂 染色体修饰剂后遗症是表原体,也就是说表原体.表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.罕见疾病是一种罕见的疾病.文字转录学 (Transcriptomics) 是一个学科.

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相关实验视频

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Screening for Functional Non-coding Genetic Variants Using Electrophoretic Mobility Shift Assay EMSA and DNA-affinity Precipitation Assay DAPA
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A Method to Study de novo Formation of Chromatin Domains
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A Method to Study de novo Formation of Chromatin Domains

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DamID-seq: Genome-wide Mapping of Protein-DNA Interactions by High Throughput Sequencing of Adenine-methylated DNA Fragments
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科学领域:

  • 表观遗传学和分子生物学
  • 基因组学和生物信息学

背景情况:

  • 表原蛋白是控制染色质结构的蛋白质,对于细胞类型特定的发育至关重要.
  • 表观基因的突变与儿科疾病和癌症有关.
  • 缺乏对表原功能多样性来源的系统分析.

研究的目的:

  • 系统地分析人类表观基因中功能多样性的来源.
  • 为了确定表原体和非表原体之间的结构和功能差异.
  • 探索表观基因相关疾病的潜在治疗点.

主要方法:

  • 使用功能基因组学数据集 (Ensembl,ENCODE,GTEx,HPO,LINCS L1000,BrainSpan) 对基因结构,同位体和蛋白质域进行比较分析.
  • 评估蛋白质复合体形成和内在无序区域 (IDR).
  • 使用L1000数据集对基因表达特征的分析和药物调节剂的识别.

主要成果:

  • 表原体比非表原体更大,拥有更多的表原体,并表现出比非表原体更大的异构形式多样性.
  • 表原体参与更多的多重体复合体,并且具有显著更大的内在无序区域 (IDR).
  • 表原体显示无处不在的表达模式,并确定了潜在的药物调节器.

结论:

  • 在异构体使用,无序域内容和结合伙伴的显著差异区分人类表原体与非表原体.
  • 这些发现有助于更好地理解表原基因在发育和疾病中的功能.
  • 这项研究为开发针对色素病变和癌症的向治疗提供了基础.