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瑞调节了由TEGDMA诱导的矩阵金属蛋白酶表达在人体芽细胞类细胞中

Paula Alejandra Baldión¹, Carlos Aldemar Díaz¹, Diego Enrique Betancourt¹

¹Departamento de Salud Oral, Facultad de Odontología, Sede Bogotá, Universidad Nacional de Colombia, Bogotá, Colombia.

Journal of biomedical materials research. Part A

|February 2, 2025

概括

瑞 (MYR) 有效地降低了暴露于树脂单体的人类芽细胞类细胞中反应性氧物种 (ROS) 和矩阵金属蛋白酶 (MMP) 的活性. 这种抗氧化作用可以保护细胞免受损伤,这表明MYR是牙科应用的潜在治疗剂.

科学领域:

生物材料科学生物材料科学
细胞生物学细胞生物学
牙科研究牙科研究

背景情况:

矩阵金属蛋白酶 (MMPs),特别是MMP-2和MMP-9,涉及树脂-牙接口降解.
牙纸细胞对树脂单体的氧化应激的反应,特别是MMP分泌,需要进一步阐明.
像Myricetin (MYR) 和proanthocyanidin (PAC) 这样的抗氧化剂显示出防止细胞外基质降解的潜力.

研究的目的:

研究Myricetin (MYR) 对MMP表达和活性的影响.
评估MYR对三乙烯糖醇二甲酸盐 (TEGDMA) 诱导的活性氧物种 (ROS) 生成的影响.
评估MYR在人类口腔细胞样细胞 (hOLC) 中对TEGDMA诱导的氧化应激的保护潜力.

主要方法:

人类的口腔细胞样细胞 (hOLCs) 使用跨井屏障模型暴露于TEGDMA.
细胞内ROS水平使用2',7'-二二化素探针进行测量.
通过RT-qPCR,ELISA和度测定量出MMP-2和MMP-9的表达和活性,无论是否接受MYR治疗.

主要成果:

在hOLC中,TEGDMA暴露显著增加了细胞内ROS和上调的MMP-2和MMP-9mRNA表达.
细胞外MMP水平及其激活能力在TEGDMA暴露后24小时显著增加.
瑞 (MYR) 治疗有效地降低了ROS的产生,并降低了MMP表达和活性在mRNA和蛋白质水平上的下调.

结论:

由于TEGDMA和MMP-2/MMP-9的表达,分泌和激活,ROS增加存在直接相关性.
瑞 (MYR) 显示出作为减轻氧化应激和控制MMP活性的治疗策略的巨大潜力.
MYR通过调节氧还原不平衡和抑制MMP介导的降解,类似于proanthocyanidin (PAC) 对hOLCs提供保护作用.

关键词:

MMP 抑制剂的使用牙牙是指牙中的牙.酶性降解酶性降解矩阵金属蛋白质酶是一种金属蛋白质.具有的烯酸化合物三乙烯糖醇二甲酸二甲酸.

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