Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Measuring Reaction Rates03:09

Measuring Reaction Rates

24.3K
Polarimetry finds application in chemical kinetics to measure the concentration and reaction kinetics of optically active substances during a chemical reaction. Optically active substances have the capability of rotating the plane of polarization of linearly polarized light passing through them—a feature called optical rotation. Optical activity is attributed to the molecular structure of substances. Normal monochromatic light is unpolarized and possesses oscillations of the electrical...
24.3K
Proteomics01:33

Proteomics

7.2K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
7.2K
Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

2.1K
Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
2.1K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

The dynamic mesoscale sink and source niches for eukaryotic phytoplankton in a subtropical gyre.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Purine bias in bacterial genes is driven by runaway transcription.

Nature microbiology·2026
Same author

AI-discovered protein fragments as generalizable regulators of biomolecular condensates.

bioRxiv : the preprint server for biology·2026
Same author

Canonical transcription termination mechanisms explain a minority of operons in cyanobacteria.

mSystems·2026
Same author

Deep Proteomic Profiles of the Antarctic Diatom Fragilariopsis Cylindrus Under Varying Iron and Manganese Conditions.

Proteomics·2026
Same author

Long-range mRNA folding shapes expression and sequence of bacterial genes.

bioRxiv : the preprint server for biology·2025
Same journal

Correction to: Keystone protist suppression triggers mesopredator release and biotic homogenization in complex soil microbial communities.

The ISME journal·2026
Same journal

Stoichiometric analysis of microbial communities links function, structure, and biomass carrying capacity.

The ISME journal·2026
Same journal

Isolation of Allocrenothrix methanica reveals distinct ecophysiologies of filamentous methanotrophs and adaptations to O2 limitation.

The ISME journal·2026
Same journal

Minimising decompression and warming during deep seawater collection increases abundance and activity of autochthonous bacteria and archaea.

The ISME journal·2026
Same journal

From strains to ecosystems: biocrust microbiomes as a new paradigm for dryland agriculture.

The ISME journal·2026
Same journal

Viral communities from long-term anaerobic alkane-oxidizing enrichments encode predicted cell surface adhesion functions.

The ISME journal·2026
查看所有相关文章

相关实验视频

Updated: May 29, 2025

Fast Enzymatic Processing of Proteins for MS Detection with a Flow-through Microreactor
09:49

Fast Enzymatic Processing of Proteins for MS Detection with a Flow-through Microreactor

Published on: April 6, 2016

7.9K

使用蛋白质和蛋白质组估计微生物反应速率.

J Scott P McCain1,2, Gregory L Britten2,3, Sean R Hackett4

  • 1Howard Hughes Medical Institute and Department of Biology, Massachusetts Institute of Technology, Cambridge, MA, United States.

The ISME journal
|February 2, 2025
PubMed
概括
此摘要是机器生成的。

在自然环境中测量微生物反应速度是很困难的. 这项研究表明,蛋白质组数据可以准确预测微生物反应速率,即使没有详细的机制信息.

关键词:
生物地质化学循环是什么代谢功能是指代谢功能.代谢建模 代谢建模蛋白质组学是指蛋白质组学.系统生物学 系统生物学

更多相关视频

An Aquatic Microbial Metaproteomics Workflow: From Cells to Tryptic Peptides Suitable for Tandem Mass Spectrometry-based Analysis
08:09

An Aquatic Microbial Metaproteomics Workflow: From Cells to Tryptic Peptides Suitable for Tandem Mass Spectrometry-based Analysis

Published on: September 15, 2015

8.7K
Selected Reaction Monitoring Mass Spectrometry for Absolute Protein Quantification
09:04

Selected Reaction Monitoring Mass Spectrometry for Absolute Protein Quantification

Published on: August 17, 2015

17.0K

相关实验视频

Last Updated: May 29, 2025

Fast Enzymatic Processing of Proteins for MS Detection with a Flow-through Microreactor
09:49

Fast Enzymatic Processing of Proteins for MS Detection with a Flow-through Microreactor

Published on: April 6, 2016

7.9K
An Aquatic Microbial Metaproteomics Workflow: From Cells to Tryptic Peptides Suitable for Tandem Mass Spectrometry-based Analysis
08:09

An Aquatic Microbial Metaproteomics Workflow: From Cells to Tryptic Peptides Suitable for Tandem Mass Spectrometry-based Analysis

Published on: September 15, 2015

8.7K
Selected Reaction Monitoring Mass Spectrometry for Absolute Protein Quantification
09:04

Selected Reaction Monitoring Mass Spectrometry for Absolute Protein Quantification

Published on: August 17, 2015

17.0K

科学领域:

  • 微生物学 微生物学
  • 生物化学 生物化学
  • 蛋白质组学是指蛋白质组学.

背景情况:

  • 微生物通过酶反应驱动环境转变.
  • 在现场量化微生物反应速率仍然是一个重大挑战.
  • 酶丰度和反应速度之间的关系尚未完全理解.

研究的目的:

  • 研究酶丰度对微生物反应速率的预测能力.
  • 确定全球蛋白质原子测量是否可以准确估计个体反应速率.
  • 探索蛋白质组作为细胞反应速率的编码器的潜力.

主要方法:

  • 从微生物培养物中收集了匹配的蛋白质和反应速率数据.
  • 使用统计分析将蛋白质组数据与测量反应速率相关联.
  • 机器学习方法被用来构建预测模型.

主要成果:

  • 仅仅酶的丰富性往往不足以预测特定的反应速率.
  • 全球蛋白质组测量能够准确预测个体反应速率 (中位数R2 = 0.78).
  • 准确的预测需要有限数量的蛋白质,并且没有先前的机械或环境背景.

结论:

  • 蛋白质组作为细胞反应速率的有效编码器.
  • 蛋白质组测量具有估计自然系统中微生物介导反应速率的潜力.
  • 这种方法为在现场研究微生物功能提供了一种新的方法.