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相关概念视频

RNA-seq03:21

RNA-seq

9.8K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
9.8K
Ribosome Profiling02:24

Ribosome Profiling

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Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
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相关实验视频

Updated: May 29, 2025

Author Spotlight: Decoding RNA Methylation's Role in Pancreatic Cancer - A Single-Base Resolution Study
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使用m-TAC-seq的基底分辨率进行RNAm5C甲基化分析的协议.

Xiaoting Zhang1, Liang Lu2, Chengqi Yi3

  • 1State Key Laboratory of Protein and Plant Gene Research, School of Life Sciences, Peking University, Beijing 100871, China.

STAR protocols
|February 2, 2025
PubMed
概括

我们开发了一种新的方法,m5C-TAC-seq,用于精确的转录组全方位RNA5甲基氨酸 (m5C) 分析. 这种没有二硫酸盐的方法在基底分辨率上准确地映射了各种RNA类型的m5C修饰.

关键词:
生物信息学是一种生物信息学.遗传学 是一个遗传学.基因组学就是基因组学.分子生物学分子生物学分子/化学探头 分子/化学探头在RNAseqqq中使用.序列分析是指进行序列分析.测序测序是指测序的时间.

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Methylated RNA Immunoprecipitation Assay to Study m5C Modification in Arabidopsis
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Methylated RNA Immunoprecipitation Assay to Study m5C Modification in Arabidopsis

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相关实验视频

Last Updated: May 29, 2025

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Methylated RNA Immunoprecipitation Assay to Study m5C Modification in Arabidopsis
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Methylated RNA Immunoprecipitation Assay to Study m5C Modification in Arabidopsis

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2D-HELS MS Seq: A General LC-MS-Based Method for Direct and de novo Sequencing of RNA Mixtures with Different Nucleotide Modifications

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科学领域:

  • 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
  • 在RNA生物学,RNA生物学.
  • 分子生物学分子生物学

背景情况:

  • RNA5-甲基细胞蛋白 (m5C) 是一种普遍存在的表体转录基因修饰.
  • m5C在调节基因表达方面发挥着重要作用.
  • 准确的m5C修饰的分析对于理解其功能影响至关重要.

研究的目的:

  • 介绍一项用于转录组全方位m5C甲基组分析的新方案.
  • 为m5C修改引入无二硫酸盐检测策略.
  • 通过使用特定的测序技术,实现m5C的基本分辨率映射.

主要方法:

  • 开发了十-十一转位 (TET) 辅助的化学标记序列 (m5C-TAC-seq).
  • 关于TET辅助化学品标签的详细协议步骤.
  • 包括图书馆建设和数据分析的程序.

主要成果:

  • m5C-TAC-seq 能够准确且可靠地检测m5C修改.
  • 该协议允许在基础分辨率下进行转录组全方位的甲基组分析.
  • 该方法适用于各种RNA物种.

结论:

  • m5C-TAC-seq为m5C分析提供了一种可靠和高效的方法.
  • 这种技术推进了对RNA修饰和基因调节的研究.
  • 该协议有助于对m5C的功能作用进行更深入的研究.