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Updated: May 29, 2025

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通过替代化学测量辅助染料结合方法对pH值和离子强度变化具有稳健性来量化白蛋白.

Marta Guembe-Garcia1, Lisa Rita Magnaghi2, Raffaela Biesuz2

  • 1Università degli Studi di Pavia, Dipartimento di Chimica, Viale Taramelli 12, Pavia 27100 Italy; Universidad de Burgos, Facultad de Ciencias, Departamento de Química, Plaza de Misael Bañuelos s/n, Burgos 09001 Spain.

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy
|February 2, 2025
PubMed
概括

新的染料结合方法提供了改进的白蛋白量化. 这些方法使用牛血清白蛋白 (BSA) 和各种染料,在广泛的pH值和离子强度范围内表现出强度.

关键词:
专辑中的专辑蛋白.化学测量 化学测量 化学测量染料结合方法 染料结合方法部分最小平方回归.主要组件分析的主要组件分析.硫甲素是硫甲素的一种.

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Determining protein-drug binding can be achieved through indirect and direct methods, each providing valuable insights into the interaction between proteins and drugs.
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The equilibrium binding constant (Kb) quantifies the strength of a protein-ligand interaction. Kb can be calculated as follows when the reaction is at equilibrium:
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