开发和描述潜在的抗菌生物复合材料:含酶的纤维素-酸材料
Carlos Eduardo Camacho-González1, Alejandro Pérez-Larios2, Cesar S Cardona-Félix3
1Tecnológico Nacional de México/I.T.Tepic, Laboratorio Nacional de Evaluación de Productos bióticos (Lanaepbi), Av. Tecnoógico No 2595, Lagos del Country, Tepic, Nayarit CP 63175, Mexico.
Colloids and surfaces. B, Biointerfaces
|February 2, 2025
概括
新型纤维素/酸盐生物复合物中含有母蛋溶酶,显示出对E. faecalis.具有显著的抗菌活性. 这些含有酶的生物复合材料为打击食品和制药应用中的细菌繁殖提供了有希望的替代方案.
科学领域:
- 生物材料科学 生物材料科学
- 微生物学 微生物学
- 聚合物化学 聚合物化学
背景情况:
- 细菌的扩散在食品和制药行业构成了重大威胁.
- 开发有效的抗微生物药物对公共卫生和产品安全至关重要.
- 莱索酶是一种抗微生物酶,具有潜力,但对于实际应用需要稳定.
研究的目的:
- 设计和表征使用修饰的纤维素/酸寡糖化物加载蛋溶酶 (CLm/AOS/Lyz) 的新型生物复合材料.
- 评估这些生物复合材料作为抗击细菌繁殖的替代品的潜力.
- 评估生物复合材料矩阵中的固定化lyszyme的稳定性和杀菌能力.
主要方法:
- 生物复合物 (CLm/AOS/Lyz) 在不同的pH值下进行合成 (3, 5, 8).
- 描述涉及富里埃变换红外光谱法 (FTIR),UV-Vis光谱法和能量分散式X射线光谱法 (EDS).
- 评估了对E. faecalis和格兰阴性细菌的抗菌活性.
主要成果:
- 证实了酶与生物复合物的成功结合,而不会改变其结构或稳定性.
- 在CLm/AOS/Lyz5中,含量和蛋白质度最高.
- 对于CLm/AOS/Lyz3和CLm/AOS/Lyz5来说,观察到可行的E. faecalis细胞的显著日志减少,与自由酶相比.
- 对于格拉姆阴性细菌来说,没有观察到可活细胞数量的显著减少.
结论:
- 含有酶的纤维素-酸生物复合物是对E. faecalis.有效的抗菌剂.
- 这些生物复合材料显示出制药和食品技术应用的潜力.
- 进一步的研究可能会探索针对更广泛的细菌的应用.
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