相关实验视频

Updated: May 29, 2025

Scanning Electron Microscopy of Macerated Tissue to Visualize the Extracellular Matrix

Scanning Electron Microscopy of Macerated Tissue to Visualize the Extracellular Matrix

Published on: June 14, 2016

纤维细胞特异的MyD88依赖信号加剧了MI心脏炎症中的炎症和心脏功能障碍

Prachi Umbarkar¹, Sultan Tousif¹, Ashish Jaiswal¹

¹Division of Cardiovascular Disease, The University of Alabama at Birmingham, AL, USA.

Biochimica et biophysica acta. Molecular basis of disease

|February 2, 2025

概括

纤维细胞特异的MyD88信号通过促进炎症,加剧了心肌梗塞 (MI) 后的心脏功能障碍. 在纤维细胞中抑制MyD88可能为治疗心脏损伤提供一种新的治疗方法.

科学领域:

心血管生物学心血管生物学
免疫学免疫学免疫学
纤维化研究纤维化研究

背景情况:

过度纤维化和慢性炎症导致心肌梗塞 (MI) 后的不良心脏重塑.
纤维细胞-免疫细胞交叉对心脏修复至关重要,但其分子机制尚不清楚.
虽然MyD88信号在免疫细胞中已知,但它在纤维细胞介导的MI病理中的作用尚未被探索.

研究的目的:

为了研究MyD88信号传递在心脏肌纤维细胞 (FBs) 在MI期间的作用.
为了阐明FB-MyD88对心脏重塑和心脏功能障碍的贡献.

主要方法:

使用可诱导的Cre系统 (Tcf21-CreERT2或Postn-CreERT2) 生成纤维细胞特异的MyD88淘汰赛 (KO) 小鼠.
在对照和KO小鼠中通过永久的LAD结合诱导MI.
评估心脏功能,炎症,纤维化和FB-免疫细胞交叉,使用共培养和化学激素分析.

主要成果:

纤维细胞特异的MyD88删除减轻了不良心脏重塑和改善心脏功能后MI.
科奥心脏显示骨髓细胞透和慢性炎症标志物的减少.
从机制上讲,FB-MyD88信号激活了纤维细胞中的NF-κB,促进了化学因子驱动的免疫细胞交叉和炎症.

结论:

来自纤维细胞的MyD88信号传递是MI诱导的慢性炎症和心脏功能障碍的关键驱动因素.
针对纤维细胞中的MyD88是一种潜在的治疗策略,可以减轻心脏病发作后的不良结果.

关键词:

纤维母细胞细胞是如何形成的纤维化纤维化纤维化炎症炎症是一种炎症.我的D8888是什么意思心肌梗塞的心脏病发作

更多相关视频

Isolation and Characterization of Adult Cardiac Fibroblasts and Myofibroblasts

Isolation and Characterization of Adult Cardiac Fibroblasts and Myofibroblasts

Published on: March 12, 2020

Suppression of Pro-fibrotic Signaling Potentiates Factor-mediated Reprogramming of Mouse Embryonic Fibroblasts into Induced Cardiomyocytes

Suppression of Pro-fibrotic Signaling Potentiates Factor-mediated Reprogramming of Mouse Embryonic Fibroblasts into Induced Cardiomyocytes

Published on: June 3, 2018

相关概念视频

Pathophysiology of Heart Failure

Pathophysiology of Heart Failure

Heart failure (HF) is a progressive syndrome involving ventricles that leads to inadequate cardiac output. It can be classified based on location and output or ejection fraction. Ejection fraction (EF) is an essential measurement in the diagnosis and surveillance of HF. Reduced EF corresponds to systolic heart failure (HFrEF). However, HF with preserved ejection fraction (HFpEF) is becoming increasingly prevalent. Also known as diastolic HF, this form of HF is related to aging. The...

Intracellular Signaling Affects Focal Adhesions

Intracellular Signaling Affects Focal Adhesions

Integrins act both as extracellular input receivers and as intracellular processing activators. As their name suggests, integrins are entirely integrated into the membrane structure. Their hydrophobic membrane-spanning regions interact with the phospholipid bilayer's hydrophobic region. These membrane receptors provide extracellular attachment sites for effectors like hormones and growth factors. They activate intracellular response cascades when their effectors are bound and active.
Some...