空气污染对精子DNA甲基化的影响
Jordan L Moore1, Seth J Parks1, Emma R James2
1Brigham Young University, Department of Cell Biology and Physiology, 4005 Life Sciences Building (LSB), Provo, UT 84602, United States.
Reproductive toxicology (Elmsford, N.Y.)
|February 2, 2025
概括
空气污染微妙地改变精子DNA甲基化,影响表观遗传健康. 这项研究揭示了特定的精子表观基因组区域易受污染诱导的变化,可能会对生殖产生影响.
科学领域:
- 环境表观遗传学环境表观遗传学
- 生殖毒理学 生殖毒理学
- 人类基因组学 人类基因组学
背景情况:
- 环境因素,包括空气污染,越来越多地被认为对精子表观基因组的影响.
- 空气污染对全球健康构成重大风险,并与各种疾病和组织甲基化变化有关.
- 了解空气污染对精子DNA甲基化的影响对于评估生殖健康和潜在的后代结果至关重要.
研究的目的:
- 研究盐湖谷季节性空气污染对精子DNA甲基化模式的影响.
- 建立暴露于空气污染和精子表观遗传健康之间的关系.
- 识别与空气污染暴露相关的精子DNA中的特定差异甲基化区域 (DMR).
主要方法:
- 在74个正常的精液样本中分析了精子DNA甲基化模式,使用了Illumina人类甲基化EPIC BeadChip阵列.
- 根据位置 (城市与农村) 和季节 (冬季与夏季) 分组样本,以比较污染暴露水平.
- 通过使用USeq软件的滑动窗口方法进行了差异甲基化分析,随后进行了确认分析和基因本体学评估.
主要成果:
- 在城市冬季 (高污染) 和城市夏季 (低污染) 样本之间确定了六个差异甲基化区域 (DMR).
- 当将城市冬季样本与农村冬季和农村夏季样本进行比较时,其中三种DMR得到证实.
- 基因本体学分析揭示了已识别的DMRs的显著关联,表明空气污染对精子表观遗传学的功能影响.
结论:
- 季节性空气污染暴露微妙地改变了人类精子中的DNA甲基化模式.
- 精子表观基因组的特定区域表现出对空气污染引起的修饰的敏感性.
- 精子中的这些表观遗传变化可能会对男性生育能力,生殖健康和潜在的后代健康产生影响.
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