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Updated: May 29, 2025

Examining the Conformational Dynamics of Membrane Proteins in situ with Site-directed Fluorescence Labeling
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在蛋白质腔内监测单个光分子的动态构造

Santiago Sosa1,2, Alan M Szalai1, Lucía F Lopez1

  • 1Centro de Investigaciones en Bionanociencias (CIBION), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Godoy Cruz 2390, Ciudad Autónoma de Buenos Aires, C1425FQD, Argentina.

Small methods
|February 3, 2025
PubMed
概括

这项研究引入了一种结合光纳米学和蛋白质工程的新方法,用于测量蛋白质内的光体方向. 这种技术揭示了生理条件下的生物系统的动态结构变化.

关键词:
这就是DNA-PAINT.分子导向的分子导向.极化分辨率显微镜的显微镜.单个分子光的光.超高分辨率的显微镜.

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Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
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