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Updated: May 29, 2025

Imaging InlC Secretion to Investigate Cellular Infection by the Bacterial Pathogen Listeria monocytogenes

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Published on: September 19, 2013

在Listeria monocytogenes期间c-Src的表征细胞间传播细胞间传播

Petra McLeod¹, Aaron S Dhanda¹, Julian A Guttman¹

¹Department of Biological Sciences, Centre for Cell Biology, Development, and Disease (C2D2), Simon Fraser University, 8888 University Dr W, Burnaby, BC, V5A 1S6, Canada.

The Journal of infectious diseases

|February 3, 2025

概括

李斯特菌在细胞之间通过动因运动性传播. 氨酸激酶c-Src对于这种细菌细胞传播至关重要,影响细菌的入侵和移动.

科学领域:

微生物学微生物学
细胞生物学细胞生物学
生物化学生物化学

背景情况:

李斯特菌 (Listeria monocytogenes) 是一种细胞内细菌,在宿主细胞之间传播.
细菌细胞间的传播依赖于基于actin的运动性和膜突起的形成.

研究的目的:

研究c-Src在Listeria monocytogenes细胞间传播中的作用.
阐明c-Src影响细菌细胞间运动的机制.

主要方法:

在Listeria monocytogenes感染期间对c-Src局部化的分析.
评估细菌在表达野生型和突变c-Src.细胞中的细胞间传播.
使用卡韦林介导的内分细胞形成途径.

主要成果:

在介导细菌进入邻近细胞的膜浸时,c-Src被丰富.
构成性活跃或非功能性c-Src突变的表达显著改变了Listeria monocytogenes细胞间传播.
c-Src活动对于有效的细胞间传播至关重要.

结论:

c-Src在Listeria monocytogenes细胞间传播中起着至关重要的作用.
氨酸激酶c-Src是影响细菌传播的关键宿主因素.
准c-Src可能会抑制L. monocytogenes的传播.

关键词:

细胞内存症是指细胞内存症.这是一种Src-依赖的酸化.细菌传播细菌传播

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