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Updated: May 29, 2025

The Development of Lyophilized Loop-mediated Isothermal Amplification Reagents for the Detection of Coxiella burnetii
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循环介导同热放大 (LAMP) 试验的可视化方法

Vinni Thekkudan Novi1, Anil Kumar Meher1, Abdennour Abbas1

  • 1Department of Bioproducts and Biosystems Engineering, University of Minnesota, St Paul, MN 55108, USA.. aabbas@umn.edu.

The Analyst
|February 3, 2025
PubMed
概括

循环介导同热放大 (LAMP) 提供快速,经济高效的核酸检测,无需复杂的设备. 本综述强调了可访问疾病诊断的视觉LAMP检测方法的进步.

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Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
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