改进了使用TrEnDi的阿片类药物,胺类药物和迷幻药物的LC-MS检测
Christian A Rosales1,2, Noah A Lepinsky1,2, Wondewossen Gebeyehu1,2
1Department of Chemistry, Carleton University, Ottawa, Ontario K1S 5B6, Canada.
Journal of the American Society for Mass Spectrometry
|February 3, 2025
概括
使用Diazomethane (TrEnDi) 的三甲基化增强剂显著提高了用于分析滥用药物的质谱学灵敏度. 这种方法改善了微量分析物的检测,并解决了复杂的物质识别挑战.
科学领域:
- 法医化学 法医化学
- 分析化学 分析化学
- 质谱测量质量谱测量
背景情况:
- 分析新型精神活性物质和走私毒品带来了重大的分析挑战.
- 气体和液体染色学与质谱学 (MS) 相结合,是药物分析的标准.
- 化学衍生物对于提高微量分析物确定灵敏度至关重要.
研究的目的:
- 引入和评估使用Diazomethane (TrEnDi) 的三甲基化增强剂,以改善基于MS的药物分析.
- 为了证明TrEnDi在提高各种滥用药物的敏感性和解决干扰方面的有效性.
- 评估TrEnDi在现实世界法医样本中的适用性.
主要方法:
- 使用TrEnDi.Di的13种滥用药物的周边甲基化.
- 使用质谱法 (MS) 进行分析,并增强灵敏度检测.
- 使用13C-同位素标记的TrEnDi试剂来解决同位素干扰.
- 采用一种非常规的近离子溶剂系统进行电喷电离.
- 在尿液矩阵中将TrEnDi应用于吗啡分析.
主要成果:
- 所有测试分析物的成功甲基化.
- 对11种化合物显著提高MS敏感性 (1.2至24.2倍).
- 改善了大多数衍生物种的逆相色谱保留.
- 使用13C-TrEnDi.使用三对受控物质之间的同位素干扰的分辨率.
- 检测到TrenDi修饰的安非他,而未修饰的安非他是不可检测的.
- 提高了2.4倍的灵敏度,并在尿液中恢复了103.1%的吗啡.
结论:
- TrEnDi是一种多功能衍生技术,可增强多发性硬化症对广泛滥用药物的敏感性.
- 该方法有助于解决等离子干扰并改善色谱行为.
- TrEnDi适用于尿液等复杂矩阵中的痕迹分析,证明了其在法医工作流程中的实际实用性.
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