人类肠道器官的内化
Rachel C Bordelon1, Madushani Herath1, Allison L Speer2
1Program in Children's Regenerative Medicine, Department of Pediatric Surgery, McGovern Medical School, The University of Texas Health Science Center at Houston.
Journal of visualized experiments : JoVE
|February 3, 2025
概括
这项研究提出了一种新的方法,通过将肠道神经系统 (ENS) 纳入人类肠道器官 (HIOs) 来设计一个功能性的生物工程小肠. 这种方法优化了祖细胞的发展,以改善肠道组织工程.
科学领域:
- 再生医学是一种再生医学.
- 发展生物学 发展生物学
- 组织工程是组织工程.
背景情况:
- 由于复杂的细胞架构和功能,创造生物工程小肠是具有挑战性的.
- 现有的人类肠道有机体 (HIO) 缺乏关键组成部分,如肠道神经系统 (ENS).
- 之前的研究已经探索了用ENS激活HIO的方法.
研究的目的:
- 开发一种独特的方法,将ENS纳入HIO衍生的生物工程小肠.
- 为了优化原始细胞的身份和发育时间,以加强ENS的整合.
- 为了创建一个更成熟和功能化的生物工程肠道模型.
主要方法:
- 人类多能干细胞 (hPSCs) 分化为HIOs和肠神经细胞 (ENCCs).
- 在早期发育阶段,ENCC与HIO共同培养 (中后肠球形).
- 共同培养在体外维持,然后移植到免疫缺陷小鼠中成熟.
主要成果:
- 开发的方法成功地将ENS集成到HIO中.
- 移植后的带有ENS的HIO (tHIO) 在体内显示出进一步的发育和成熟.
- 早期的共同培养策略最大限度地暴露于发育线索,促进更成熟的肠道形态学.
结论:
- 这种综合方法为产生具有功能性ENS的生物工程小肠提供了一个有希望的策略.
- 该方法优化了发育时间和祖细胞利用,以改善组织工程结果.
- 这种技术的进一步发展可以促进肠道疾病的治疗.
相关概念视频
Anatomy of the Intestines
Although digestion of proteins, carbohydrates, and lipids may begin in the stomach, it is completed in the intestine. The absorption of nutrients, water, and electrolytes from food and drink also occurs in the intestine. The intestines can be divided into two structurally distinct organs—the small and large intestines.
Small Intestines
The small intestine is an ~7 meter-long tube with an inner diameter of just 2.5 cm. Since most nutrients are absorbed here, the inner lining of the small...
Small Intestines
The small intestine is an ~7 meter-long tube with an inner diameter of just 2.5 cm. Since most nutrients are absorbed here, the inner lining of the small...
Major Organs of the Digestive System
The digestive system is responsible for the ingestion of food, secretion of enzymes, mixing and digesting food, absorption of the nutrients and defecation. The human digestive system consists of two major parts: the gastrointestinal tract and the accessory digestive organs.
Gastrointestinal tract:
Gastrointestinal tract:
Gross Anatomy of the Stomach
The human stomach is a vital part of the digestive system, performing multiple functions. It is located within the peritoneum, a serous membrane that lines the abdominal cavity. The stomach plays a central role in processing food substances and interacts with other digestive organs through coordinated digestive processes. The stomach has a characteristic J-shape and is divided into four main regions. The cardia is the first section where the esophagus connects to the stomach and is the entry...
Histology of the Small Intestine
The small intestine exhibits a unique histological structure that significantly enhances its function in digestion and nutrient absorption. These structures include circular folds, villi, and various specialized cells that collectively facilitate the digestion of food.
The intestinal lining features transverse folds called circular folds, each housing fingerlike projections known as intestinal villi. These villi are covered by a layer of simple columnar epithelium, also referred to as...
The intestinal lining features transverse folds called circular folds, each housing fingerlike projections known as intestinal villi. These villi are covered by a layer of simple columnar epithelium, also referred to as...
Histology of the Large Intestine
The large intestine, a vital component of the gastrointestinal tract, is structured with four main layers: the mucosa, submucosa, muscularis, and serosa. Each layer performs a distinct role in facilitating the smooth functioning of the large intestine.
The innermost mucosa layer comprises simple columnar epithelium, lamina propria, and muscularis mucosae. This layer is primarily populated with absorptive cells, tasked with water absorption, and goblet cells, responsible for secreting mucus to...
The innermost mucosa layer comprises simple columnar epithelium, lamina propria, and muscularis mucosae. This layer is primarily populated with absorptive cells, tasked with water absorption, and goblet cells, responsible for secreting mucus to...
Intestinal Obstruction I: Introduction
Intestinal obstruction is a partial or complete blockage of the small or large intestine that disrupts the normal flow of intestinal contents through the lumen. This interruption impairs digestion, absorption, and fluid balance, and may lead to serious complications if not treated promptly.Mechanical ObstructionMechanical obstruction occurs when a physical blockage prevents intestinal contents from passing, arising from within the lumen or the bowel wall, or from external compression.Adhesions,...


