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从Moringa oleifera叶片中识别基酸作为使用亲和选择-质谱法调节子聚合的调节剂

Magali Silva de Amorim¹, Manuela Amaral-do-Nascimento², Vanessa Gisele Pasqualotto Severino³

¹Instituto de Química, Departamento de Química Orgânica, BioCrom, Universidade Federal Fluminense, 24210-141 Niterói, RJ, Brazil.

|February 3, 2025

概括

莫林加油提取物含有抑制子蛋白聚合的化合物,这是致命的神经退行性子疾病的关键因素. 基和新基酸显示出显著的抗胺活性,提供潜在的新疗法.

科学领域:

神经科学和药理学神经科学和药理学
自然产品化学自然产品化学

背景情况:

子疾病是致命的神经退行性疾病,由错误折叠的子蛋白 (PrP^Sc) 引起.
目前对性病的治疗方法是无效的,需要开发新的治疗策略.

研究的目的:

为了研究Moringa oleifera提取物的潜力,作为可以调节蛋白聚合的化合物的来源.
为了确定M. oleifera*中负责抗活性的特定生物活性化合物.

主要方法:

使用实时震动诱导转换 (RT-QuIC) 试验进行聚抑制概况.
采用亲和选择质谱法 (AS-MS) 来识别原油提取物中的活性化合物.
使用IC₅₀值量化抑制活性.

主要成果:

确定了基和新基酸作为子聚合的强有力的抑制剂.
这些化合物表现出显著的抗活性,IC₅₀值分别为64.41 ± 12.12 μM和35.34 ± 7.09 μM.
基和新基酸抑制了PrP^C到PrP^Sc的转化和分解的预制PrP^Sc纤维素 *in vitro*.

结论:

来自Moringa oleifera的基和新基酸显示出对病和其他粉原性疾病的治疗潜力.
AS-MS是一种有效的工具,用于快速识别来自自然来源的生物活性化合物.
需要进一步的体内研究来评估这些化合物的治疗疗效.

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