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Generation of Alginate Microspheres for Biomedical Applications

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Published on: August 12, 2012

富科伊丹酸盐和硫酸酸盐微粒诱导不同的凝血,炎症和纤维性反应

Kalaiyarasi Vasuthas^1,2, Joachim Sebastian Kjesbu³, Alessandro Brambilla^2,4,5

¹Centre of Molecular Inflammation Research (CEMIR), NTNU, Norway.

Materials today. Bio

|February 3, 2025

概括

富科伊丹酸盐微珠比硫酸酸盐微珠更容易引发炎症和纤维化. 等离子体活性和凝血之间的平衡,以及ECM降解蛋白酶,预测细胞治疗材料中的纤维化耐药性.

科学领域:

生物材料科学生物材料科学
免疫学免疫学免疫学
蛋白质组学是指蛋白质组学.

背景情况:

酸微珠在细胞疗法中用于免疫保护.
硫酸酸盐微珠可以减少纤维化和炎症.
的多糖化合物富科伊丹 (Fucoidan) 尚未在微珠形式对其宿主反应进行评估.

研究的目的:

为了比较宿主对福科伊丹酸盐微珠的反应与硫酸酸盐微珠的反应.
评估蛋白质吸附,细胞因子概况和周囊细胞过度生长 (PFO).
为了确定预测纤维化耐药性的生物标志物.

主要方法:

人体全血和血测定蛋白质活性.
在C57BL/6JRj小鼠中评估外囊过度生长 (PFO).
液体染色学-双重质谱学 (LC-MS/MS) 蛋白质组学用于蛋白质模式映射.

主要成果:

富科伊丹阿尔金酸盐微珠激活补充和凝固,诱导更高的PFO.
这两种微珠类型都诱导了等离子体活性,但富可伊丹基酸盐微珠显示出明显的细胞因子概况 (增加了MCP-1,IL-8,IFN-γ;减少了RANTES,Eotaxin,PDGF-BB,TGF-β).
富科伊丹酸盐微珠丰富了细胞外矩阵因子,而硫酸酸盐微珠则有利于ECM降解蛋白酶.

结论:

宿主对富科伊丹和硫酸藻酸盐微珠的反应存在显著差异.
等离子体活性与凝血的比率是纤维化耐药性的关键指标.
在材料表面上丰富ECM降解蛋白酶对于缓解纤维化至关重要.

关键词:

降解ECM的蛋白质酶.纤维化解是一种纤维化解.水凝是一种水凝.在 LC/MS-Proteomics 中使用.硫酸多糖化合物硫酸多糖化合物表面的表面表面的表面

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