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Updated: Jun 25, 2026

An Ex vivo Assay to Study Candida albicans Hyphal Morphogenesis in the Gastrointestinal Tract

An Ex vivo Assay to Study Candida albicans Hyphal Morphogenesis in the Gastrointestinal Tract

Published on: July 1, 2020

菌株背景与第7号染色体无倍化相互作用,以确定Candida albicans中的共生和毒性表型

Abhishek Mishra^1,2, Norma V Solis³, Siobhan M Dietz⁴

¹Department of Microbiology, The Ohio State University, Columbus, OH, 43210, USA.

bioRxiv : the preprint server for biology

|February 3, 2025

概括

基因组变化,如Candida albicans的第7染色体三症,影响其毒性和殖民. 这些影响因真菌菌株背景而有很大差异,挑战了以前的假设.

科学领域:

微生物学微生物学
遗传学是一个遗传学.
病变的发生和发病.

背景情况:

*Candida albicans* 是一种人类的真菌病原体,以基因组可塑性而闻名,包括体积.
染色体三症,特别是染色体7 (Chr7) 是常见的,并且与显著的表型变化有关,例如增强的小鼠肠道殖民.
之前对Chr7三症的研究主要使用SC5314参考菌株,不清楚不同遗传背景的表型的保存情况.

研究的目的:

调查Chr7三症在*Candida albicans*的毒性和殖民中的作用,无论是SC5314参考菌株还是529L.commensal样口腔候群病菌株.
为了确定Chr7三症相关的表型是否在不同的*C. albicans*遗传背景中得到保存.
在真菌表型上探索Chr7三症,菌株背景和同类标识 (AAB与ABB) 之间的相互作用.

主要方法:

在*C. albicans*株529L和SC5314.4中对Chr7三症的比较分析.
对*体外*线材的评估.
在小鼠模型中对口腔殖民的评估.
在小鼠模型中评估系统感染期间的毒性.
对基因剂量效应的分析,特别是对于NRG1*.

主要成果:

在529L和SC5314菌株背景中,Chr7三症对殖民和毒性产生不同的影响.
在529L菌株中,Chr7三症增强了口腔殖民,但在SC5314.4中没有.
在SC5314中,Chr7三症减少了系统性毒性,但在529L中没有;SC5314中的AAB Chr7三症导致了毒性.
在两种菌株中,*In vitro* 丝是唯一一个由 Chr7 三合症持续改变的表型.
增加*NRG1*基因剂量并没有复制大多数观察到的Chr7三症表型.

结论:

状体,特别是Chr7三症,与*Candida albicans*的遗传背景变异复杂地相互作用.
体积的表型结果并不普遍保存,并且取决于特定的菌株和宿主利基.
基于SC5314菌株,目前对体积在*C. albicans*病变发生过程中的作用的理解需要改进,以解释遗传多样性.

关键词:

这是一种Candida Candida.没有积症.染色体同类的染色体同类这是一种共生主义 (commensalism).菌株多样性菌株多样性

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