Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

6.9K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
6.9K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Modulation of Oncogenic KRAS Signaling by Branched Actin-driven Cell Membrane Protrusions.

Research square·2026
Same author

Altair-dvOPM: an open-access platform for large-field three-dimensional tissue imaging.

bioRxiv : the preprint server for biology·2026
Same author

Modulation of Oncogenic KRAS Signaling by Branched Actin-driven Cell Membrane Protrusions.

bioRxiv : the preprint server for biology·2026
Same author

An epithelial morphogenetic program for maximal urine concentration.

Nature communications·2026
Same author

Leptin Receptor <sup>+</sup> cells create a perisinusoidal niche for thrombopoiesis in the bone marrow by synthesizing CXCL14.

bioRxiv : the preprint server for biology·2026
Same author

Author Correction: PHGDH heterogeneity potentiates cancer cell dissemination and metastasis.

Nature·2026
Same journal

Layered social competition coordinates reproductive hierarchy formation in ants.

bioRxiv : the preprint server for biology·2026
Same journal

Combination epigenetic-targeted therapy increases the immunogenicity of poorly immunogenic sarcomas.

bioRxiv : the preprint server for biology·2026
Same journal

Loss of LanC-like proteins delays post-injury regeneration of aging skeletal muscles.

bioRxiv : the preprint server for biology·2026
Same journal

Integrative Transfer Network: Deep Transfer Learning Across Populations and Prediction Targets.

bioRxiv : the preprint server for biology·2026
Same journal

Confidence-supported label-free metabolic imaging with FPhaS phase autofluorescence microscopy.

bioRxiv : the preprint server for biology·2026
Same journal

Sequence-encoded autoinhibition couples mRNA decapping activity to phase separation.

bioRxiv : the preprint server for biology·2026
查看所有相关文章

相关实验视频

Updated: May 29, 2025

Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers
10:07

Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers

Published on: April 9, 2014

10.0K

具有亚细胞分辨率的特征驱动全组织成像.

Jinlong Lin, Zach Marin, Xiaoding Wang

    bioRxiv : the preprint server for biology
    |February 3, 2025
    PubMed
    概括
    此摘要是机器生成的。

    我们开发了多尺度清除组织轴扫光板显微镜 (MCT-ASLM),用于跨尺度成像完整的组织. 这种先进的显微镜平台将亚细胞细节连接到整个组织结构,有助于生物发现.

    更多相关视频

    Single-Cell Resolution Three-Dimensional Imaging of Intact Organoids
    10:40

    Single-Cell Resolution Three-Dimensional Imaging of Intact Organoids

    Published on: June 5, 2020

    16.0K
    Three-dimensional Imaging of Bacterial Cells for Accurate Cellular Representations and Precise Protein Localization
    06:33

    Three-dimensional Imaging of Bacterial Cells for Accurate Cellular Representations and Precise Protein Localization

    Published on: October 29, 2019

    9.9K

    相关实验视频

    Last Updated: May 29, 2025

    Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers
    10:07

    Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers

    Published on: April 9, 2014

    10.0K
    Single-Cell Resolution Three-Dimensional Imaging of Intact Organoids
    10:40

    Single-Cell Resolution Three-Dimensional Imaging of Intact Organoids

    Published on: June 5, 2020

    16.0K
    Three-dimensional Imaging of Bacterial Cells for Accurate Cellular Representations and Precise Protein Localization
    06:33

    Three-dimensional Imaging of Bacterial Cells for Accurate Cellular Representations and Precise Protein Localization

    Published on: October 29, 2019

    9.9K

    科学领域:

    • 生物成像成像技术
    • 显微镜技术的使用方法
    • 组织生物学 组织生物学

    背景情况:

    • 了解亚细胞事件和组织范围的现象之间的联系至关重要.
    • 现有的成像方法在复杂的生物系统中面临规模和分辨率的局限性.

    研究的目的:

    • 为了引入多尺度清除组织轴扫光板显微镜 (MCT-ASLM).
    • 为了使完整的组织从厘米尺度到亚微米分辨率的成像.
    • 为了促进对等级组织和复杂的生物过程的研究.

    主要方法:

    • 开发了MCT-ASLM,将大视野成像与高分辨率功能相结合.
    • 使用轴扫光片显微镜来清除组织样本.
    • 启用了人类引导和自主成像模式.

    主要成果:

    • 在微米级分辨率下,MCT-ASLM可以捕获高达21毫米的视野.
    • 该平台可以过渡到亚微米 (~300 nm) 的同位素分辨率用于定向成像.
    • 在绘制神经元电路图,可视化脏内置和检查瘤微环境方面展示了应用.

    结论:

    • MCT-ASLM提供了一种通用工具,可以跨越亚细胞和组织水平的尺度.
    • 这项技术推进了对生物样本等级组织的研究.
    • MCT-ASLM提供了一种强大的方法来研究当地生物事件如何影响全球现象.