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相关概念视频

RNA Splicing01:32

RNA Splicing

55.9K
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
55.9K
Pre-mRNA Processing: RNA Splicing01:36

Pre-mRNA Processing: RNA Splicing

5.2K
5.2K
Alternative RNA Splicing02:18

Alternative RNA Splicing

20.9K
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
20.9K
Chromatin Structure and RNA Splicing02:41

Chromatin Structure and RNA Splicing

2.7K
2.7K
RNA Editing02:23

RNA Editing

8.9K
RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...
8.9K
RNA Structure01:19

RNA Structure

4.6K
The basic structure of RNA consists of a string of ribonucleotides attached by phosphodiester bonds. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA) involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three...
4.6K

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相关实验视频

Updated: May 29, 2025

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells
10:06

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells

Published on: April 26, 2017

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用于RNA拼接预测和设计的生成建模.

Di Wu1, Natalie Maus1, Anupama Jha2

  • 1Department of Computer and Information Science, School of Engineering, University of Pennsylvania.

bioRxiv : the preprint server for biology
|February 3, 2025
PubMed
概括

我们开发了TrASPr+BOS,这是一种使用生成模型和贝叶斯优化来预测和设计用于组织特定替代拼接 (AS) 的RNA的AI工具. 这种方法增强了对基因调节的理解,并有助于治疗的发展.

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Using the E1A Minigene Tool to Study mRNA Splicing Changes
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相关实验视频

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Using the E1A Minigene Tool to Study mRNA Splicing Changes
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Using the E1A Minigene Tool to Study mRNA Splicing Changes

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A Reporter Based Cellular Assay for Monitoring Splicing Efficiency
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科学领域:

  • 分子生物学分子生物学
  • 生物信息学是一种生物信息学.
  • 人工智能的人工智能

背景情况:

  • 替代拼接 (AS) 对于组织特异性基因调节至关重要,并与疾病有关.
  • 拼接缺陷可以导致各种病理,突出需要预测和操纵工具.
  • 了解和控制AS为新的治疗策略提供了潜力.

研究的目的:

  • 介绍TrASPr+BOS,一个新的生成AI模型与贝叶斯优化相结合.
  • 预测和设计RNA序列,以实现特定的取决于组织的替代拼接结果.
  • 推进基于RNA的治疗方法的设计,并了解基因调节.

主要方法:

  • 开发了TrASPr,一个多变压器生成AI模型,能够处理多种AS事件并将其推广到新的条件.
  • 使用TrASPr作为一个神话来生成标记数据用于训练贝叶斯对拼接 (BOS) 算法的优化.
  • 采用BOS来设计RNA序列用于特定条件的拼接调制.

主要成果:

  • 在预测组织特异性拼接方面,TrASPr+BOS显著优于现有方法.
  • 在组织特定的AUPRC (精度回忆曲线下的区域) 中达到2.4倍的增强.
  • 使用dCas13技术识别和验证了新的组织特异性拼接变异和监管元素.

结论:

  • TrASPr+BOS提供了一种强大而准确的方法,用于预测和设计用于组织特定替代拼接的RNA.
  • 该模型成功地捕获了关键的组织特异性调节元素,推进了RNA治疗领域.
  • 这种方法为研究基因调节和开发向治疗的研究人员提供了一种多功能工具.