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相关概念视频

Animal Mitochondrial Genetics02:59

Animal Mitochondrial Genetics

7.4K
Among all the organelles in an animal cell, only mitochondria have their own independent genomes. Animal mitochondrial DNA is a double-stranded, closed-circular molecule with around 20,000 base pairs. Mitochondrial DNA is unique in that one of its two strands, the heavy, or H, -strand is guanine rich, whereas the complementary strand is cytosine rich and called the light, or L, -strand. Compared to nuclear DNA, mitochondrial DNA has a very low percentage of non-coding regions and is marked by...
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Mismatch Repair01:20

Mismatch Repair

4.8K
Organisms are capable of detecting and fixing nucleotide mismatches that occur during DNA replication. This sophisticated process requires identifying the new strand and replacing the erroneous bases with correct nucleotides. Mismatch repair is coordinated by many proteins in both prokaryotes and eukaryotes.
The Mutator Protein Family Plays a Key Role in DNA Mismatch Repair
The human genome has more than 3 billion base pairs of DNA per cell. Prior to cell division, that vast amount of genetic...
4.8K
RNA Editing02:23

RNA Editing

8.9K
RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...
8.9K

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相关实验视频

Updated: May 29, 2025

Functional Assessment of BRCA1 variants using CRISPR-Mediated Base Editors
09:22

Functional Assessment of BRCA1 variants using CRISPR-Mediated Base Editors

Published on: February 28, 2021

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MitoEdit:用于优化mtDNA基编辑和预测旁观者效应的管道.

Devansh Shah, Kelly McCastlain, Ti-Cheng Chang

    bioRxiv : the preprint server for biology
    |February 3, 2025
    PubMed
    概括

    MitoEdit是一个新的工具,可以预测线粒体DNA (mtDNA) 基编辑中的意外编辑,简化对mtDNA突变和相关疾病的研究.

    科学领域:

    • 基因组学就是基因组学.
    • 分子生物学分子生物学
    • 生物信息学是一种生物信息学.

    背景情况:

    • 线粒体DNA (mtDNA) 突变与遗传性呼吸系统疾病有关.
    • 身体mtDNA突变在慢性疾病和癌症中的作用需要进一步研究.
    • 目前的mtDNA基编辑工具面临的挑战是预测意外的"旁观者"编辑.

    研究的目的:

    • 开发一种计算工具,用于在mtDNA基编辑中预测旁观者编辑.
    • 为了优化对mtDNA突变研究的目标窗口的识别.
    • 为了减少 mtDNA 基编辑构造的实验性试错开发.

    主要方法:

    • 开发了MitoEdit,这是一种用于mtDNA基编辑的新型计算工具.
    • MitoEdit分析DNA序列,以预测潜在的旁观者编辑及其功能影响.
    • 该工具包含经验基础编辑模式和TALE绑定序列.

    主要成果:

    • MitoEdit准确地预测了大部分在 silico.
    • 该工具可以识别最佳的目标窗口,并减少对实证测试的需求.
    • MitoEdit可以自动预测线粒体DNA中的基数编辑.

    更多相关视频

    Genotyping Single Nucleotide Polymorphisms in the Mitochondrial Genome by Pyrosequencing
    07:24

    Genotyping Single Nucleotide Polymorphisms in the Mitochondrial Genome by Pyrosequencing

    Published on: February 10, 2023

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    A Nonsequencing Approach for the Rapid Detection of RNA Editing
    08:50

    A Nonsequencing Approach for the Rapid Detection of RNA Editing

    Published on: April 21, 2022

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    相关实验视频

    Last Updated: May 29, 2025

    Functional Assessment of BRCA1 variants using CRISPR-Mediated Base Editors
    09:22

    Functional Assessment of BRCA1 variants using CRISPR-Mediated Base Editors

    Published on: February 28, 2021

    5.4K
    Genotyping Single Nucleotide Polymorphisms in the Mitochondrial Genome by Pyrosequencing
    07:24

    Genotyping Single Nucleotide Polymorphisms in the Mitochondrial Genome by Pyrosequencing

    Published on: February 10, 2023

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    A Nonsequencing Approach for the Rapid Detection of RNA Editing
    08:50

    A Nonsequencing Approach for the Rapid Detection of RNA Editing

    Published on: April 21, 2022

    2.5K

    结论:

    • MitoEdit 是第一个用于预测线粒体DNA中基数编辑的自动化工具.
    • 这种工具可以加速对mtDNA突变及其在疾病中的作用的研究.
    • MitoEdit是科学界可以免费使用的.